Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Our Abpromise guarantee covers the use of ab28030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).|
|ICC/IF||Use at an assay dependent dilution.|
FunctionDsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic during interphase. Relocates to the nucleus as cells enter mitosis.
- Information by UniProt
- F2 antibody
- Factor 2 antibody
- Factor2 antibody
Anti-TTF2 antibody (ab28030) at 1/1000 dilution + HeLa nuclear extract
rabbit anti-sheep peroxidase linked at 1/10000 dilution
Predicted band size: 130 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?