Overview

  • Product name

    Anti-TTF2 antibody [EPR12756]
    See all TTF2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12756] to TTF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TTF2 aa 50-150. The exact sequence is proprietary.
    Database link: Q9UNY4

  • Positive control

    • Daudi and HeLa cell line lysates; Jurkat cells; Human adenocarcinoma of endometrium tissue; Human pancreas tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
IHC-P 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic during interphase. Relocates to the nucleus as cells enter mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • F2 antibody
    • Factor 2 antibody
    • Factor2 antibody
    • HuF 2 antibody
    • HuF2 antibody
    • Human factor 2 antibody
    • Lodestar homolog antibody
    • Lodestar protein antibody
    • RNA polymerase II termination factor antibody
    • Transcription release factor 2 antibody
    • Transcription termination factor 2 antibody
    • Transcription termination factor RNA polymerase II antibody
    • TTF2 antibody
    • TTF2_HUMAN antibody
    see all

Images

  • All lanes : Anti-TTF2 antibody [EPR12756] (ab181116) at 1/5000 dilution

    Lane 1 : Daudi cell line lysate
    Lane 2 : HeLa cell line lysate
    Lane 3 : Jurkat cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 130 kDa

  • Immunohistochemical analysis of paraffin embedded Human adenocarcinoma of endometrium tissue labeling TTF2 with ab181116 at 1/250. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

  • Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling TTF2 with ab181116 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

  • Immunofluorescent analysis of acetone-fixed Jurkat cells labeling TTF2 with ab181116 at 1/100 (green) dilution. Goat anti rabbit IgG (Alexa Fluor® 488) was used for secondary antibody staining at a dilution of 1/200. Counter stain: Dapi (blue)

  • Flow cytometric analysis of  2% paraformaldehyde-fixed Jurkat cells labeling TTF2 with ab181116 at 1/60 dilution, followed by Goat anti rabbit IgG (FITC) at 1/150 dilution (pink). Rabbit monoclonal IgG was used as Isotype control (green).

References

ab181116 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
ChIP
Sample
Mouse Cell lysate - nuclear (Erythroblast cell line)
Negative control
ALAS2
Specification
Erythroblast cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 32 minute(s) and 0 second(s)
Specification of the cross-linking agent: EGS 30 min + formaldehyde 2 mi
Positive control
KIT TSS

Dr. Marion Dubuissez

Verified customer

Submitted Jun 14 2017

Application
Immunoprecipitation
Sample
Mouse Cell lysate - nuclear (Erythroblast cell line)
Total protein in input
50 µg
Immuno-precipitation step
Protein G
Specification
Erythroblast cell line

Dr. Marion Dubuissez

Verified customer

Submitted Jul 29 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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