Key features and details
- Rabbit polyclonal to TTK/Mps1
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TTK/Mps1 antibody
See all TTK/Mps1 primary antibodies
DescriptionRabbit polyclonal to TTK/Mps1
Specificityab24227 reacts with TTK (MPS1) protein. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
- Hela cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to TTK immobilized on solid support.
Our Abpromise guarantee covers the use of ab24227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/2500 - 1/25000. Predicted molecular weight: 95 kDa.|
|IP||Use at an assay dependent dilution.|
FunctionPhosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
Tissue specificityPresent in rapidly proliferating cell lines.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
- Information by UniProt
- cancer/testis antigen 96 antibody
- CT96 antibody
- Dual specificity protein kinase TTK antibody
All lanes : Anti-TTK/Mps1 antibody (ab24227)
Lane 1 : HeLa cell lyste at 15µg and ab at 0.033ug/ml
Lane 2 : HeLa cell lyste at 50ug and ab at 0.033ug/ml
Lanes 3-4 : HeLa cell lyste at 50ug and ab at 0.33ug/ml
Lanes 5-7 : HeLa cell lyste at 2mg and ab at 1ug/2mg lysate
Developed using the ECL technique.
Predicted band size: 95 kDa
Observed band size: 95 kDa
ECL exposure time:
B: 15 second
C: 30 second
ICC/IF image of TTK/Mps1 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24227, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab24227 has not yet been referenced specifically in any publications.