Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)

Reviews (8)Q&A (1)References (20)
Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
  • Immunoprecipitation - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
  • Immunocytochemistry/ Immunofluorescence - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
  • Flow Cytometry - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
  • Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)

Key features and details

  • Mouse monoclonal [N1] to TTK/Mps1 - BSA free
  • Suitable for: Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-TTK/Mps1 antibody [N1] - BSA free
    See all TTK/Mps1 primary antibodies

  • Description

    Mouse monoclonal [N1] to TTK/Mps1 - BSA free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Recombinant fragment within Human TTK/Mps1 aa 1-900. The exact sequence is proprietary. (catalytically inactive - D663A)

  • Epitope

    This antibody recognizes an epitope in the N-terminal region of Mps1.

  • Positive control

    • In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF (1)
Use a concentration of 1 µg/ml.
IP (2)
Use at an assay dependent concentration.
WB (5)
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). NOT SUITABLE for blocking with BSA. Block in 5% milk for 1 hour.
Notes
Flow Cyt
Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 1 µg/ml.
IP
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). NOT SUITABLE for blocking with BSA. Block in 5% milk for 1 hour.

Target

  • Function

    Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.

  • Tissue specificity

    Present in rapidly proliferating cell lines.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • cancer/testis antigen 96 antibody
      • CT96 antibody
      • Dual specificity protein kinase TTK antibody
      • ECSTY kinase antibody
      • ESK antibody
      • FLJ38280 antibody
      • hMPS1 antibody
      • Monopolar Spindle 1 Like 1 antibody
      • MPH1 antibody
      • Mps 1 antibody
      • MPS1L1 antibody
      • Phosphotyrosine Picked Threonine Kinase antibody
      • Phosphotyrosine picked threonine-protein kinase antibody
      • PYT antibody
      • RP3-357D13.2 antibody
      • ttk antibody
      • TTK Protein Kinase antibody
      • TTK_HUMAN antibody
      see all

    Images

    • Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      All lanes : Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 4 : Jurkat nuclear extract lysate (ab14844)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 95 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?


      Exposure time: 8 minutes


      NOTE: MILK was used to block non-specific binding, rather than BSA.
    • Immunoprecipitation - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Immunoprecipitation - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)

      TTK/Mps1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Mouse monoclonal to Mps1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab11108.
      Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
      Band: 100kDa: TTK/Mps1 

    • Immunocytochemistry/ Immunofluorescence - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Immunocytochemistry/ Immunofluorescence - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      ICC/IF image of ab11108 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab11108, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • Flow Cytometry - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Flow Cytometry - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Overlay histogram showing HeLa cells stained with ab11108 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11108, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 10 µg/ml + Recombinant human TTK/Mps1 protein (ab89589) at 0.01 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 95 kDa


      Exposure time: 8 minutes


      ab11108 recognizes the full length tagged recombinant TTK/Mps1 protein (ab89589) which has an expected molecular weight of 130 kDa.

    References (20)

    Publishing research using ab11108? Please let us know so that we can cite the reference in this datasheet.

    ab11108 has been referenced in 20 publications.

    • Fang Q  et al. The essential roles of Mps1 in spermatogenesis and fertility in mice. Cell Death Dis 12:531 (2021). PubMed: 34031364
    • Manohar S  et al. The Insulin Receptor Adaptor IRS2 is an APC/C Substrate That Promotes Cell Cycle Protein Expression and a Robust Spindle Assembly Checkpoint. Mol Cell Proteomics 19:1450-1467 (2020). PubMed: 32554797
    • Gui P  et al. Mps1 dimerization and multisite interactions with Ndc80 complex enable responsive spindle assembly checkpoint signaling. J Mol Cell Biol 12:486-498 (2020). PubMed: 32219319
    • Zhang L  et al. Mitotic checkpoint kinase Mps1/TTK predicts prognosis of colon cancer patients and regulates tumor proliferation and differentiation via PKCa/ERK1/2 and PI3K/Akt pathway. Med Oncol 37:5 (2019). PubMed: 31720873
    • Raghavendra A  et al. Expression of MAGE-A and NY-ESO-1 cancer/testis antigens is enriched in triple-negative invasive breast cancers. Histopathology 73:68-80 (2018). PubMed: 29465777
    View all Publications for this product

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mps1 antibody [N1]

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Ms. Chelsea Backer

    Verified customer

    Submitted Apr 14 2009

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