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    ttkmps1-antibody-n1-bsa-free-ab11108.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Other
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Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)

  • Datasheet
  • SDS
Reviews (8)Q&A (1)References (17)

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Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
  • Immunoprecipitation - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
  • Immunocytochemistry/ Immunofluorescence - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
  • Flow Cytometry - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
  • Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)

Key features and details

  • Mouse monoclonal [N1] to TTK/Mps1 - BSA free
  • Suitable for: Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-TTK/Mps1 antibody [N1] - BSA free
    See all TTK/Mps1 primary antibodies
  • Description

    Mouse monoclonal [N1] to TTK/Mps1 - BSA free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment within Human TTK/Mps1 aa 1-900. The exact sequence is proprietary. (catalytically inactive - D663A)

  • Epitope

    This antibody recognizes an epitope in the N-terminal region of Mps1.
  • Positive control

    • In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    N1
  • Myeloma

    PAIB3Ag81
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant human TTK/Mps1 protein (ab89589)

Applications

Our Abpromise guarantee covers the use of ab11108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). NOT SUITABLE for blocking with BSA. Block in 5% milk for 1 hour.

Target

  • Function

    Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
  • Tissue specificity

    Present in rapidly proliferating cell lines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession P33981 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7272 Human
    • Omim: 604092 Human
    • SwissProt: P33981 Human
    • Unigene: 169840 Human
    • Alternative names

      • cancer/testis antigen 96 antibody
      • CT96 antibody
      • Dual specificity protein kinase TTK antibody
      • ECSTY kinase antibody
      • ESK antibody
      • FLJ38280 antibody
      • hMPS1 antibody
      • Monopolar Spindle 1 Like 1 antibody
      • MPH1 antibody
      • Mps 1 antibody
      • MPS1L1 antibody
      • Phosphotyrosine Picked Threonine Kinase antibody
      • Phosphotyrosine picked threonine-protein kinase antibody
      • PYT antibody
      • RP3-357D13.2 antibody
      • ttk antibody
      • TTK Protein Kinase antibody
      • TTK_HUMAN antibody
      see all

    Images

    • Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      All lanes : Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 4 : Jurkat nuclear extract lysate (ab14844)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 95 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?


      Exposure time: 8 minutes


      NOTE: MILK was used to block non-specific binding, rather than BSA.
    • Immunoprecipitation - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Immunoprecipitation - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)

      TTK/Mps1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Mouse monoclonal to Mps1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab11108.
      Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
      Band: 100kDa: TTK/Mps1 

    • Immunocytochemistry/ Immunofluorescence - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Immunocytochemistry/ Immunofluorescence - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      ICC/IF image of ab11108 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab11108, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • Flow Cytometry - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Flow Cytometry - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Overlay histogram showing HeLa cells stained with ab11108 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11108, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)
      Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 10 µg/ml + Recombinant human TTK/Mps1 protein (ab89589) at 0.01 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 95 kDa


      Exposure time: 8 minutes


      ab11108 recognizes the full length tagged recombinant TTK/Mps1 protein (ab89589) which has an expected molecular weight of 130 kDa.

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (17)

    Publishing research using ab11108? Please let us know so that we can cite the reference in this datasheet.

    ab11108 has been referenced in 17 publications.

    • Gui P  et al. Mps1 dimerization and multisite interactions with Ndc80 complex enable responsive spindle assembly checkpoint signaling. J Mol Cell Biol 12:486-498 (2020). PubMed: 32219319
    • Raghavendra A  et al. Expression of MAGE-A and NY-ESO-1 cancer/testis antigens is enriched in triple-negative invasive breast cancers. Histopathology 73:68-80 (2018). PubMed: 29465777
    • Choi M  et al. TC Mps1 12, a novel Mps1 inhibitor, suppresses the growth of hepatocellular carcinoma cells via the accumulation of chromosomal instability. Br J Pharmacol 174:1810-1825 (2017). PubMed: 28299790
    • Zhang J  et al. Downregulation of tyrosine threonine kinase inhibits tumor growth via G2/M arrest in human endometrioid endometrial adenocarcinoma. Tumour Biol 39:1010428317712444 (2017). PubMed: 28718377
    • Wild T  et al. The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C Activity. Cell Rep 14:1829-40 (2016). WB ; Human . PubMed: 26904940
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Western blot abreview for Anti-Mps1 antibody [N1]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (8)
    Sample
    Human Cell lysate - whole cell (Human colon carcinoma cells)
    Specification
    Human colon carcinoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Mar 11 2014

    Western blot abreview for Anti-Mps1 antibody [N1]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (8)
    Sample
    Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells)
    Specification
    Chinese Hamster Ovary cells
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Feb 27 2014

    Immunoprecipitation abreview for Anti-Mps1 antibody [N1]

    Excellent
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A/G
    Sample
    Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells)
    Specification
    Chinese Hamster Ovary cells
    Total protein in input
    50 µg
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Feb 27 2014

    Immunoprecipitation abreview for Anti-Mps1 antibody [N1]

    Excellent
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A/G
    Sample
    Human Cell lysate - whole cell (Human colon carcinoma cells)
    Specification
    Human colon carcinoma cells
    Total protein in input
    50 µg
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Feb 27 2014

    Western blot abreview for Anti-Mps1 antibody [N1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    10 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 15°C
    Read More

    Ms. AeLee Jeong

    Verified customer

    Submitted Apr 12 2013

    Question

    are there perhaps any news regarding my inquiry?
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 29 2013

    Answer

    Thank you for your email.



    Lab has confirmed that the recommended concentration is actually 1ug/ml; the 10ug/ml has been added in error which will be corrected soon. With the new recommended dilutions, this antibody will be suitable for100 tests which is the lower limit of acceptable tests with one vial.

    I hope this information will be helpful. Should you have any other inquiry please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jan 29 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mps1 antibody [N1]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Ms. Chelsea Backer

    Verified customer

    Submitted Apr 14 2009

    Western blot abreview for Anti-Mps1 antibody [N1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    10 µg
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 23 2009

    Western blot abreview for Anti-Mps1 antibody [N1]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 08 2005

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