Key features and details
- Mouse monoclonal [OTI13E9] to TTK/Mps1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-TTK/Mps1 antibody [OTI13E9]
See all TTK/Mps1 primary antibodies
DescriptionMouse monoclonal [OTI13E9] to TTK/Mps1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
Recombinant fragment corresponding to Human TTK/Mps1 aa 189-464. (NP_003309) produced in E.coli.
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Database link: P33981
- WB: K562 and Raji cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 97 kDa.|
FunctionPhosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
Tissue specificityPresent in rapidly proliferating cell lines.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
- Information by UniProt
- cancer/testis antigen 96 antibody
- CT96 antibody
- Dual specificity protein kinase TTK antibody
All lanes : Anti-TTK/Mps1 antibody [OTI13E9] (ab236525) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 97 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236525 has not yet been referenced specifically in any publications.