Key features and details
- Rabbit polyclonal to TTLL4 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TTLL4 antibody - C-terminal
See all TTLL4 primary antibodies
DescriptionRabbit polyclonal to TTLL4 - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
- HeLa, 293T, and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab186045 was affinity purified using an epitope specific to TTLL4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab186045 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 133 kDa.|
FunctionPolyglutamylase which preferentially modifies beta-tubulin and nucleosome assembly proteins NAP1 and NAP2. Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step.
Sequence similaritiesBelongs to the tubulin--tyrosine ligase family.
Contains 1 TTL domain.
Cellular localizationCell projection > cilium. Cytoplasm > cytoskeleton > cilium basal body. Located in cilia. In some cells, also found in basal bodies.
- Information by UniProt
- TTLL4 antibody
- TTLL4_HUMAN antibody
- Tubulin polyglutamylase TTLL4 antibody
- Tubulin--tyrosine ligase-like protein 4 antibody
Detection of TTLL4 by Western Blot of Immunprecipitate.
Lane 1: ab186045 at 1µg/ml staining TTLL4 in 293T whole cell lysate immunoprecipitated using ab186045 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection: Chemiluminescence with exposure time of 3 minutes.
All lanes : Anti-TTLL4 antibody - C-terminal (ab186045) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 133 kDa
Exposure time: 3 minutes
ab186045 has not yet been referenced specifically in any publications.