Product nameAnti-TTLL5 antibody
DescriptionRabbit polyclonal to TTLL5
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Orangutan
- HeLa, Jurkat and 293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187697 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 144 kDa.|
FunctionPolyglutamylase which preferentially modifies alpha-tubulin. Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step (By similarity). Increases the effects of NCOA2 in glucocorticoid receptor-mediated repression and induction and in androgen receptor-mediated induction.
Tissue specificityWidely expressed with highest levels in heart and skeletal muscle and low levels in other tissues.
Sequence similaritiesBelongs to the tubulin--tyrosine ligase family.
Contains 1 TTL domain.
Cellular localizationCell projection > cilium. Cytoplasm > cytoskeleton > cilium basal body. Nucleus. Cytoplasm. In ciliated cells, located in both cilia and basal bodies (By similarity). In osteosarcoma cells, found in both cytoplasm and nucleus in the absence of steroid but located exclusively in the nucleus in the presence of steroid.
- Information by UniProt
- KIAA0998 antibody
- SRC1 and TIF2-associated modulatory protein antibody
- STAMP antibody
Detection of TTLL5 by Western Blot of Immunprecipitate.
Lane 1: ab187697 1µg/ml labeling TTLL5 in HeLa whole cell lysate immunoprecipitated using ab187697 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 3 minutes.
All lanes : Anti-TTLL5 antibody (ab187697) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 144 kDa
Exposure time: 3 minutes
ab187697 has not yet been referenced specifically in any publications.