Key features and details
- Rabbit polyclonal to TTYH3
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TTYH3 antibody
DescriptionRabbit polyclonal to TTYH3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human TTYH3 aa 425-475. The exact sequence is proprietary. (GeneID 80727).
Database link: Q9C0H2
- WB: Malme-3M, HeLa and HEK-293T cell lysate. IP: Malme-3M cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab240580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 50 µg/mg of lysate.|
RelevanceProbable large-conductance Ca2+-activated chloride channel. May play a role in Ca2+ signal transduction.
Cellular localizationCell membrane; Multi-pass membrane protein
- KIAA1691 antibody
- hTTY3 antibody
- Protein tweety homolog 3 antibody
All lanes : Anti-TTYH3 antibody (ab240580) at 1/1000 dilution
Lane 1 : Malme-3M cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Exposure time: 30 seconds
TTYH3 was immunoprecipitated from Malme-3M whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer, using ab240580 at 50 μl per reaction. For western blotting, ab240580 was used at 1/100 dilution.
Lane 1: ab240580 used for IP at 50 µl per reaction.
Lane 2: Control IgG.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240580 has not yet been referenced specifically in any publications.