Recombinant
RabMAb

Recombinant Anti-TUB 1 antibody [EPR8241] (ab131034)

Overview

  • Product name

    Anti-TUB 1 antibody [EPR8241]
    See all TUB 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8241] to TUB 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of Human TUB 1 (UniProt P50607).

  • Positive control

    • SH-SY5Y, Saos-2, C6, L6, and Neuro-2a cell lysates; Human astrocytoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to membranes containing phosphatidylinositol 4,5-bisphosphate. Can bind DNA (in vitro). May contribute to the regulation of transcription in the nucleus. Could be involved in the hypothalamic regulation of body weight (By similarity). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages.
    • Sequence similarities

      Belongs to the TUB family.
    • Cellular localization

      Cytoplasm. Nucleus. Secreted. Cell membrane. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.
    • Information by UniProt
    • Database links

    • Alternative names

      • F10B5.4 antibody
      • rd5 antibody
      • TUB 1 antibody
      • TUB antibody
      • TUB_HUMAN antibody
      • Tubby homologue antibody
      • Tubby protein homolog 1 antibody
      • Tubby protein homolog antibody
      see all

    Images

    • All lanes : Anti-TUB 1 antibody [EPR8241] (ab131034) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : Saos-2 cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : L6 cell lysate
      Lane 5 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 56 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labelling TUB 1 with ab131034 antibody at 1/100 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab131034 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131034.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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