Product nameAnti-TUB 1 antibody [EPR8241]
See all TUB 1 primary antibodies
DescriptionRabbit monoclonal [EPR8241] to TUB 1
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues near the N-terminus of Human TUB 1 (UniProt P50607).
- SH-SY5Y, Saos-2, C6, L6, and Neuro-2a cell lysates; Human astrocytoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.43 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionFunctions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to membranes containing phosphatidylinositol 4,5-bisphosphate. Can bind DNA (in vitro). May contribute to the regulation of transcription in the nucleus. Could be involved in the hypothalamic regulation of body weight (By similarity). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages.
Sequence similaritiesBelongs to the TUB family.
Cellular localizationCytoplasm. Nucleus. Secreted. Cell membrane. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.
- Information by UniProt
- F10B5.4 antibody
- rd5 antibody
- TUB 1 antibody
All lanes : Anti-TUB 1 antibody [EPR8241] (ab131034) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : L6 cell lysate
Lane 5 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labelling TUB 1 with ab131034 antibody at 1/100 dilution.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131034 has not yet been referenced specifically in any publications.