Key features and details
- Rabbit polyclonal to TUBA1B
- Suitable for: WB, IHC-P, IHC - Wholemount
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-TUBA1B antibody
See all TUBA1B primary antibodies
DescriptionRabbit polyclonal to TUBA1B
Tested applicationsSuitable for: WB, IHC-P, IHC - Wholemountmore details
Species reactivityReacts with: Zebrafish
The details of the immunogen for this antibody are not available.
- WB: Zebrafish brain and eye extracts. IHC-P: Zebrafish tissue. IHC-Wm: 2 days post fertilization zebrafish embryo.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab233169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
|IHC-P||1/100 - 1/1000.|
|IHC - Wholemount||1/100 - 1/500.|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Sequence similaritiesBelongs to the tubulin family.
modificationsUndergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Alpha tubulin ubiquitous antibody
- Alpha-tubulin ubiquitous antibody
- K alpha 1 antibody
All lanes : Anti-TUBA1B antibody (ab233169) at 1/2000 dilution
Lane 1 : Zebrafish brain extract
Lane 2 : Zebrafish eye extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
2 days post fertilization zebrafish embryo stained for TUBA1B using ab233169 at 1/100 dilution in wholemount immunohistochemical analysis.
Paraffin-embedded zebrafish tissue stained for TUBA1B using ab233169 at 1/300 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233169 has not yet been referenced specifically in any publications.