Overview

  • Product name

  • Description

    Rabbit polyclonal to TUBA4A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
    Predicted to work with: Sheep, Cow, Xenopus laevis, Chimpanzee
  • Immunogen

    Recombinant fragment within Human TUBA4A (intracellular). The exact sequence is proprietary.
    Database link: P68366

  • Positive control

    • WB: HeLa, Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12, Rat2, Jurkat, Raji, HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900)/extract; HeLa cytosolic fraction; Drosophila whole cell lysate/extract. IHC-P: Mouse brain tissue; rat brain tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/20000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha tubulin 1 antibody
    • Alpha-tubulin 1 antibody
    • FLJ30169 antibody
    • H2 alpha antibody
    • TBA4A_HUMAN antibody
    • Testis specific alpha tubulin antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA 4A antibody
    • TUBA1 antibody
    • Tuba4a antibody
    • Tubulin alpha 1 (testis specific) antibody
    • Tubulin alpha 1 antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha 4a antibody
    • Tubulin alpha 4A chain antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2 alpha antibody
    • Tubulin H2-alpha antibody
    see all

Images

  • All lanes : Anti-TUBA4A antibody (ab228701) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TUBA4A (green) using ab228701  at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded rat brain tissue stained for TUBA4A with ab228701 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-TUBA4A antibody (ab228701) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract at 20 µg
    Lane 2 : HEK-293T whole cell lysate/extract at 10 µg
    Lane 3 : HEK-293T whole cell lysate/extract at 5 µg

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • Anti-TUBA4A antibody (ab228701) at 1/2000 dilution + Drosophila lysate/extract at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • All lanes : Anti-TUBA4A antibody (ab228701) at 1/10000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa cytosolic fraction
    Lane 3 : HeLa nuclear fraction

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • All lanes : Anti-TUBA4A antibody (ab228701) at 1/10000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • All lanes : Anti-TUBA4A antibody (ab228701) at 1/20000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • All lanes : Anti-TUBA4A antibody (ab228701) at 1/20000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C8D30 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • Paraffin-embedded mouse brain tissue stained for TUBA4A with ab228701 at 1/500 dilution in immunohistochemical analysis.

References

ab228701 has not yet been referenced specifically in any publications.

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