Recombinant
RabMAb

Recombinant Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

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Overview

  • Product name

    Anti-TUBA4A antibody [EPR13477(B)]
    See all TUBA4A primary antibodies

  • Description

    Rabbit monoclonal [EPR13477(B)] to TUBA4A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Flow Cyt, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TUBA4A aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P68366

  • Positive control

    • Molt-4, A431, Jurkat, HeLa and K562 cell lysates; Human testis and Human uterus tissues; A431 cells; K562 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13477(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/250.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

  • Sequence similarities

    Belongs to the tubulin family.

  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Alpha tubulin 1 antibody
    • Alpha-tubulin 1 antibody
    • FLJ30169 antibody
    • H2 alpha antibody
    • TBA4A_HUMAN antibody
    • Testis specific alpha tubulin antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA 4A antibody
    • TUBA1 antibody
    • Tuba4a antibody
    • Tubulin alpha 1 (testis specific) antibody
    • Tubulin alpha 1 antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha 4a antibody
    • Tubulin alpha 4A chain antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2 alpha antibody
    • Tubulin H2-alpha antibody
    see all

Images

  • Western blot - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Western blot - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    All lanes : Anti-TUBA4A antibody [EPR13477(B)] (ab177479) at 1/1000 dilution

    Lane 1 : Molt-4 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 50 kDa

  • Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1X PBS (negative control) using ab177479, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TUBA4A with ab177479 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TUBA4A with ab177479 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunofluorescent analysis of A431 cells labeling TUBA4A with ab177479 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Flow Cytometry - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Flow cytometric analysis of permeabilized K562 cells labeling TUBA4A with ab177479 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

References

ab177479 has not yet been referenced specifically in any publications.

Publishing research using ab177479? Please let us know so that we can cite the reference in this datasheet.

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