Product nameAnti-TUBA4A antibody [EPR13477(B)]
See all TUBA4A primary antibodies
DescriptionRabbit monoclonal [EPR13477(B)] to TUBA4A
Tested applicationsSuitable for: IP, Flow Cyt, IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TUBA4A aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P68366
- Molt-4, A431, Jurkat, HeLa and K562 cell lysates; Human testis and Human uterus tissues; A431 cells; K562 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/250.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/100 - 1/250.|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Alpha tubulin 1 antibody
- Alpha-tubulin 1 antibody
- FLJ30169 antibody
All lanes : Anti-TUBA4A antibody [EPR13477(B)] (ab177479) at 1/1000 dilution
Lane 1 : Molt-4 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa
Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1X PBS (negative control) using ab177479, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TUBA4A with ab177479 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TUBA4A with ab177479 at 1/100 dilution.
Immunofluorescent analysis of A431 cells labeling TUBA4A with ab177479 at 1/100 dilution (red). DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized K562 cells labeling TUBA4A with ab177479 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
ab177479 has not yet been referenced specifically in any publications.