Anti-TUBB2A + TUBB2B antibody (ab155311)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TUBB2A + TUBB2B antibody -
Description
Rabbit polyclonal to TUBB2A + TUBB2B -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Pig, Monkey
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-237 of Human TUBB2A (UniProt Q13885).
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Positive control
- 293T, A431, Jurkat, Raji, NIH-3T3 and mouse brain lysates; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1% BSA, 78% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 50 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Cellular localization
TUBB2A: Cytoplasm > cytoskeleton. TUBB2B: Cytoplasm, cytoskeleton. -
Database links
- Entrez Gene: 347733 Human
- Entrez Gene: 7280 Human
- Entrez Gene: 22151 Mouse
- Entrez Gene: 73710 Mouse
- Entrez Gene: 291081 Rat
- Entrez Gene: 498736 Rat
- Omim: 612850 Human
- SwissProt: P09203 Chicken
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Images
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Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/3000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-TUBB2A + TUBB2B antibody (ab155311)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling beta II Tubulin with ab155311 at 1/200 dilution. Lower panel co-stained with Hoechst 33342 .
References
ab155311 has not yet been referenced specifically in any publications.
