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Tags & Cell Markers Subcellular Markers Cytoskeleton Microtubules
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Anti-TUBB2A + TUBB2B antibody (ab155311)

  • Datasheet
  • SDS
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Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)
  • Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)
  • Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)
  • Immunocytochemistry/ Immunofluorescence - Anti-TUBB2A + TUBB2B antibody (ab155311)

Key features and details

  • Rabbit polyclonal to TUBB2A + TUBB2B
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TUBB2A + TUBB2B antibody
  • Description

    Rabbit polyclonal to TUBB2A + TUBB2B
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-237 of Human TUBB2A (UniProt Q13885).

  • Positive control

    • 293T, A431, Jurkat, Raji, NIH-3T3 and mouse brain lysates; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Mouse brain tissue lysate - total protein (ab30151)
    • NIH/3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Mouse
Human
All applications
Rat
Chicken
Cow
Pig
Monkey
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Cellular localization

    TUBB2A: Cytoplasm > cytoskeleton. TUBB2B: Cytoplasm, cytoskeleton.
  • Database links

    • Entrez Gene: 347733 Human
    • Entrez Gene: 7280 Human
    • Entrez Gene: 22151 Mouse
    • Entrez Gene: 73710 Mouse
    • Entrez Gene: 291081 Rat
    • Entrez Gene: 498736 Rat
    • Omim: 612850 Human
    • SwissProt: P09203 Chicken
    • SwissProt: Q6B856 Cow
    • SwissProt: Q13885 Human
    • SwissProt: Q9BVA1 Human
    • SwissProt: Q7TMM9 Mouse
    • SwissProt: Q9CWF2 Mouse
    • SwissProt: P02554 Pig
    • SwissProt: P85108 Rat
    • SwissProt: Q3KRE8 Rat
    • Unigene: 300701 Human
    • Unigene: 654543 Human
    • Unigene: 379227 Mouse
    • Unigene: 422827 Mouse
    • Unigene: 472121 Mouse
    • Unigene: 225893 Rat
    • Unigene: 37849 Rat
    see all

Images

  • Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)
    Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)
    Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/3000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)
    Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)
    Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)
    Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311)
    Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-TUBB2A + TUBB2B antibody (ab155311)
    Immunocytochemistry/ Immunofluorescence - Anti-TUBB2A + TUBB2B antibody (ab155311)
    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling beta II Tubulin with ab155311 at 1/200 dilution. Lower panel co-stained with Hoechst 33342 .

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab155311? Please let us know so that we can cite the reference in this datasheet.

ab155311 has not yet been referenced specifically in any publications.

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