Key features and details
- Rabbit polyclonal to TUBB6
- Suitable for: IHC-P, WB
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-TUBB6 antibody
DescriptionRabbit polyclonal to TUBB6
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Zebrafish
Recombinant fragment within Zebrafish TUBB6 aa 194-466. The exact sequence is proprietary.
Database link: 558552
- Adult zebrafish whole cell lysate and zebrafish tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab210087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Also suggested as antigen retrieval, heat-mediated Tris-EDTA buffer (pH8.0)
|WB||1/500 - 1/3000. Predicted molecular weight: 53 kDa.|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Sequence similaritiesBelongs to the tubulin family.
DomainThe highly acidic C-terminal region may bind cations such as calcium.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Entrez Gene: 558552 Zebrafish
- tubulin beta-6 chain-like antibody
- class V beta-tubulin antibody
- HsT1601 antibody
ab210087 has not yet been referenced specifically in any publications.