Overview

  • Product name

    Anti-TUBB6 antibody
  • Description

    Rabbit polyclonal to TUBB6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Recombinant fragment within Zebrafish TUBB6 aa 194-466. The exact sequence is proprietary.
    Database link: 558552

  • Positive control

    • Adult zebrafish whole cell lysate and zebrafish tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab210087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Also suggested as antigen retrieval, heat-mediated Tris-EDTA buffer (pH8.0)

WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • tubulin beta-6 chain-like antibody
    • class V beta-tubulin antibody
    • HsT1601 antibody
    • TBB6_HUMAN antibody
    • TUBB-5 antibody
    • TUBB6 antibody
    • Tubulin beta 6 chain antibody
    • tubulin beta 6 class V antibody
    • tubulin beta class V antibody
    • tubulin beta MGC4083 antibody
    • Tubulin beta-6 antibody
    • Tubulin beta-6 chain antibody
    see all

Images

  • Anti-TUBB6 antibody (ab210087) at 1/1000 dilution + adult zebrafish whole cell lysate at 30 µg

    Predicted band size: 53 kDa



    7.5% SDS PAGE

  • Immunohistochemical analysis of paraffin-embedded zebrafish tissue, labeling TUBB6 using ab210087 at a 1/300 dilution.

References

ab210087 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab210087.
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