• Product name

    Anti-Tubby antibody
  • Description

    Rabbit polyclonal to Tubby
  • Host species

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Tubby.

    Read Abcam's proprietary immunogen policy (Peptide available as ab47529.)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Spinal Cord, Mouse Brain, Mouse Testis, Mouse Spinal Cord, Rat Brain and Rat Dorsal Root Ganglion.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab46943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).


  • Relevance

    Tubby is a bipartite transcription factor. It may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, which functions as a membrane bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis.
  • Cellular localization

    Cytoplasm. Nucleus. Secreted. Cell membrane. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.
  • Database links

  • Alternative names

    • Mouse tubby homologue antibody
    • rd5 antibody
    • Retinal degeneration 5 antibody
    • TUB antibody
    • Tubby (mouse) homolog antibody
    • tubby antibody
    • Tubby homolog (mouse) antibody
    • Tubby homologue antibody
    • Tubby protein homolog antibody
    see all


  • All lanes : Anti-Tubby antibody (ab46943) at 1 µg/ml

    Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate
    Lane 5 : Brain (Rat) Tissue Lysate - normal tissue
    Lane 6 : Rat Dorsal Root Ganglion

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

  • IHC image of Tubby staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab46943, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


ab46943 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


I am very sorry to keeping you waiting.

I just got the information from my lab colleagues.

They confirmed the mouse strain used is CD-1 and the animals used are surplus animals over 8 weeks old.

I hope this helps. Please do not hesitate to contact us back for further information.

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Thank you for contacting us.

I am sorry you are experiencing difficulties with this antibody.

The lysates were prepared using RIPA buffer and phosphatase inhibitors. The gel was run under reducing conditions, and the blocking was perform in 5%BSA/0.1%TBST for one hour with gentle rocking. Antibodies are incubated overnight at 4 degrees C in blocking buffer (5%BSA/0.1%TBST). The membrane was washed with 0.1% TBST 3x10minutes at RT.

The procedure was a standard WB protocol as described in our online protocol:


I hope you find this information useful. If you had any further problems using this antibody please do not hesitate to contact us back. All of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

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