Overview

  • Product name

    Anti-Tuberin antibody - N-terminal
    See all Tuberin primary antibodies
  • Description

    Rabbit polyclonal to Tuberin - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Tuberin (N terminal). The exact sequence is proprietary. 14 amino acid peptide from near the amino terminus. NCBI Accession No. NP_000539.2.
    Database link: P49815

  • Positive control

    • Human pancreas tissue; mouse L1210 cells and cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab189304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 201 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.
  • Tissue specificity

    Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
  • Involvement in disease

    Defects in TSC2 are the cause of tuberous sclerosis type 2 (TSC2) [MIM:613254]. TSC2 is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. It is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
    Defects in TSC2 are a cause of lymphangioleiomyomatosis (LAM) [MIM:606690]. LAM is a progressive and often fatal lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. It affects almost exclusively young women and can occur as an isolated disorder or in association with tuberous sclerosis complex.
  • Sequence similarities

    Contains 1 Rap-GAP domain.
  • Post-translational
    modifications

    Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
    Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation.
  • Cellular localization

    Cytoplasm. Membrane. At steady state found in association with membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ43106 antibody
    • LAM antibody
    • OTTHUMP00000158940 antibody
    • OTTHUMP00000198394 antibody
    • OTTHUMP00000198395 antibody
    • PPP1R160 antibody
    • Protein phosphatase 1, regulatory subunit 160 antibody
    • TSC complex subunit 2 antibody
    • tsc2 antibody
    • TSC2_HUMAN antibody
    • TSC4 antibody
    • TSC4 gene, formerly antibody
    • TSC4, formerly antibody
    • Tuberin antibody
    • Tuberous sclerosis 2 antibody
    • Tuberous sclerosis 2 protein antibody
    • Tuberous sclerosis 2 protein homolog antibody
    see all

Images

  • Lane 1 : Anti-Tuberin antibody - N-terminal (ab189304) at 2 µg/ml
    Lane 2 : Anti-Tuberin antibody - N-terminal (ab189304) at 4 µg/ml

    All lanes : mouse L1210 cells

    Predicted band size: 201 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling Tuberin using ab189304 at 5 µg/ml.

  • Immunofluorescence analysis of mouse L1210 cells, labeling Tuberin using ab189304 at 20 µg/ml.

  • Immunocytochemistry analysis of mouse L1210 cells, labeling Tuberin using ab189304 at 10 µg/ml.

References

ab189304 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (central nervous system)
Permeabilization
Yes - triton 0.5%
Specification
central nervous system
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Mar 14 2017

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