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RecombinantRabMAb

Recombinant Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962)

  • Datasheet
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Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962)
  • Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962)
  • Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1062Y] to Tuberin (phospho S939)
  • Suitable for: WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Knockout
Human TSC2 (Tuberin) knockout A549 cell line (ab277867)

View more associated products

Overview

  • Product name

    Anti-Tuberin (phospho S939) antibody [EP1062Y]
    See all Tuberin primary antibodies
  • Description

    Rabbit monoclonal [EP1062Y] to Tuberin (phospho S939)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-Fr,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 3T3 cell lysates (untreated or treated with insulin). WB: SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1062Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 201 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 201 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-Fr,IHC-P or IP.

Target

  • Function

    In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.
  • Tissue specificity

    Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
  • Involvement in disease

    Defects in TSC2 are the cause of tuberous sclerosis type 2 (TSC2) [MIM:613254]. TSC2 is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. It is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
    Defects in TSC2 are a cause of lymphangioleiomyomatosis (LAM) [MIM:606690]. LAM is a progressive and often fatal lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. It affects almost exclusively young women and can occur as an isolated disorder or in association with tuberous sclerosis complex.
  • Sequence similarities

    Contains 1 Rap-GAP domain.
  • Post-translational
    modifications

    Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
    Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation.
  • Cellular localization

    Cytoplasm. Membrane. At steady state found in association with membranes.
  • Target information above from: UniProt accession P49815 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7249 Human
    • Entrez Gene: 22084 Mouse
    • Entrez Gene: 24855 Rat
    • Omim: 191092 Human
    • SwissProt: P49815 Human
    • SwissProt: Q61037 Mouse
    • SwissProt: P49816 Rat
    • Unigene: 90303 Human
    • Unigene: 30435 Mouse
    • Unigene: 5875 Rat
    see all
  • Alternative names

    • FLJ43106 antibody
    • LAM antibody
    • OTTHUMP00000158940 antibody
    • OTTHUMP00000198394 antibody
    • OTTHUMP00000198395 antibody
    • PPP1R160 antibody
    • Protein phosphatase 1, regulatory subunit 160 antibody
    • TSC complex subunit 2 antibody
    • tsc2 antibody
    • TSC2_HUMAN antibody
    • TSC4 antibody
    • TSC4 gene, formerly antibody
    • TSC4, formerly antibody
    • Tuberin antibody
    • Tuberous sclerosis 2 antibody
    • Tuberous sclerosis 2 protein antibody
    • Tuberous sclerosis 2 protein homolog antibody
    see all

Images

  • Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962)
    Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962)
    All lanes : Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962) at 1/1000 dilution

    Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates
    Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 201 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?


    Exposure time: 90 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    This image is derived from purified batch.

  • Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962)
    Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962)
    All lanes : Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962) at 1/10000 dilution

    Lane 1 : 3T3 cell lysate untreated
    Lane 2 : 3T3 cell lysate treated with insulin

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 201 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?

  • Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962)
    Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (3)

Publishing research using ab52962? Please let us know so that we can cite the reference in this datasheet.

ab52962 has been referenced in 3 publications.

  • Mallela K  et al. Oncogenic role of MiR-130a in oral squamous cell carcinoma. Sci Rep 11:7787 (2021). PubMed: 33833339
  • Tang L  et al. Electroacupuncture Upregulated Ghrelin in Rats with Functional Dyspepsia via AMPK/TSC2/Rheb-Mediated mTOR Inhibition. Dig Dis Sci 65:1689-1699 (2020). PubMed: 31863340
  • Shiono M  et al. The G1556S-type tuberin variant suppresses tumor formation in tuberous sclerosis 2 mutant (Eker) rats despite its deficiency in mTOR inhibition. Oncogene 27:6690-7 (2008). PubMed: 18695678

Customer reviews and Q&As

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