RecombinantRabMAb

Recombinant Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)

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Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
  • Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
  • Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1062Y] to Tuberin (phospho S939) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free
    See all Tuberin primary antibodies

  • Description

    Rabbit monoclonal [EP1062Y] to Tuberin (phospho S939) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247344 is the carrier-free version of ab52962. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247344 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 201 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 201 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-Fr,IHC-P or IP.

    • Target

    • Function

      In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.

    • Tissue specificity

      Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.

    • Involvement in disease

      Defects in TSC2 are the cause of tuberous sclerosis type 2 (TSC2) [MIM:613254]. TSC2 is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. It is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
      Defects in TSC2 are a cause of lymphangioleiomyomatosis (LAM) [MIM:606690]. LAM is a progressive and often fatal lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. It affects almost exclusively young women and can occur as an isolated disorder or in association with tuberous sclerosis complex.

    • Sequence similarities

      Contains 1 Rap-GAP domain.

    • Post-translational
      modifications

      Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
      Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation.

    • Cellular localization

      Cytoplasm. Membrane. At steady state found in association with membranes.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • FLJ43106 antibody
      • LAM antibody
      • OTTHUMP00000158940 antibody
      • OTTHUMP00000198394 antibody
      • OTTHUMP00000198395 antibody
      • PPP1R160 antibody
      • Protein phosphatase 1, regulatory subunit 160 antibody
      • TSC complex subunit 2 antibody
      • tsc2 antibody
      • TSC2_HUMAN antibody
      • TSC4 antibody
      • TSC4 gene, formerly antibody
      • TSC4, formerly antibody
      • Tuberin antibody
      • Tuberous sclerosis 2 antibody
      • Tuberous sclerosis 2 protein antibody
      • Tuberous sclerosis 2 protein homolog antibody
      see all

    Images

    • Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
      Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
      All lanes : Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962) at 1/1000 dilution

      Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
      Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates
      Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates. Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 201 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?


      Exposure time: 90 seconds


      This data was developed using ab52962, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      This image is derived from purified batch.

    • Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
      Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
      All lanes : Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962) at 1/10000 dilution

      Lane 1 : 3T3 cell lysate untreated
      Lane 2 : 3T3 cell lysate treated with insulin

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 201 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?



      This data was developed using ab52962, the same antibody clone in a different buffer formulation.

       

    • Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
      Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247344? Please let us know so that we can cite the reference in this datasheet.

    ab247344 has not yet been referenced specifically in any publications.

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