Recombinant
RabMAb

Recombinant Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] - BSA and Azide free (ab247850)

Overview

  • Product name

    Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] - BSA and Azide free
    See all Tuberin primary antibodies
  • Description

    Rabbit monoclonal [EPR1060(2)] to Tuberin (phospho T1462) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects Tuberin phosphorylated at Threonine 1462.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Tuberin (phospho T1462). The exact sequence is proprietary.

  • General notes

    ab247850 is the carrier-free version of ab109403 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247850 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 201 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.
    • Tissue specificity

      Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
    • Involvement in disease

      Defects in TSC2 are the cause of tuberous sclerosis type 2 (TSC2) [MIM:613254]. TSC2 is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. It is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
      Defects in TSC2 are a cause of lymphangioleiomyomatosis (LAM) [MIM:606690]. LAM is a progressive and often fatal lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. It affects almost exclusively young women and can occur as an isolated disorder or in association with tuberous sclerosis complex.
    • Sequence similarities

      Contains 1 Rap-GAP domain.
    • Post-translational
      modifications

      Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
      Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation.
    • Cellular localization

      Cytoplasm. Membrane. At steady state found in association with membranes.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ43106 antibody
      • LAM antibody
      • OTTHUMP00000158940 antibody
      • OTTHUMP00000198394 antibody
      • OTTHUMP00000198395 antibody
      • PPP1R160 antibody
      • Protein phosphatase 1, regulatory subunit 160 antibody
      • TSC complex subunit 2 antibody
      • tsc2 antibody
      • TSC2_HUMAN antibody
      • TSC4 antibody
      • TSC4 gene, formerly antibody
      • TSC4, formerly antibody
      • Tuberin antibody
      • Tuberous sclerosis 2 antibody
      • Tuberous sclerosis 2 protein antibody
      • Tuberous sclerosis 2 protein homolog antibody
      see all

    References

    ab247850 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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