Anti-TUBGCP2 antibody [GCP2-01] (ab140225)
Key features and details
- Mouse monoclonal [GCP2-01] to TUBGCP2
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-TUBGCP2 antibody [GCP2-01] -
Description
Mouse monoclonal [GCP2-01] to TUBGCP2 -
Host species
Mouse -
Specificity
ab140225 recognizes TUBGCP2 and phosphorylated TUBGCP2. -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to amino acids 2-194 of Mouse TUBGCP2 with a proprietary tag.
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Positive control
- Flow Cyt: HeLa cells. WB: Jurkat, HeLa, HEK293T, U87-MG all under reducing conditions.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab140225 is purified from cell culture supernatant by protein A affinity chromatography to a purity of > 95% (by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
GCP2-01 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 0.1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 103 kDa.
reducing conditions. |
Notes |
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Flow Cyt
Use 0.1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 103 kDa. reducing conditions. |
Target
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Function
Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the GCP family. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 10844 Human
- SwissProt: Q9BSJ2 Human
- Unigene: 523370 Human
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Alternative names
- 1700022B05Rik antibody
- A disintegrin and metalloprotease domain 8 antibody
- Adam8 antibody
see all
Images
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Overlay histogram showing HeLa cells stained with ab140225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140225, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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Western blotting analysis of TUBGCP2 using ab140225 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of ab140225 followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GCP2 at approximately 95 kDa.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab140225 has been referenced in 1 publication.
- Dráberová E et al. Overexpression and Nucleolar Localization of ?-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma. J Neuropathol Exp Neurol 74:723-42 (2015). Human . PubMed: 26079448