Overview

  • Product name

    Anti-TUBGCP2 antibody [GCP2-01]
    See all TUBGCP2 primary antibodies
  • Description

    Mouse monoclonal [GCP2-01] to TUBGCP2
  • Host species

    Mouse
  • Specificity

    ab140225 recognizes TUBGCP2 and phosphorylated TUBGCP2.
  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Recombinant fragment corresponding to amino acids 2-194 of Mouse TUBGCP2 with a proprietary tag.

Properties

Applications

Our Abpromise guarantee covers the use of ab140225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Predicted molecular weight: 103 kDa. Under reducing conditions
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the GCP family.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1700022B05Rik antibody
    • A disintegrin and metalloprotease domain 8 antibody
    • Adam8 antibody
    • Gamma ring complex protein 103 kDa antibody
    • Gamma tubulin complex component 2 antibody
    • Gamma tubulin complex protein 2 antibody
    • Gamma-ring complex protein 103 kDa antibody
    • Gamma-tubulin complex component 2 antibody
    • GCP 2 antibody
    • GCP-2 antibody
    • GCP2 antibody
    • GCP2_HUMAN antibody
    • h103p antibody
    • hGCP2 antibody
    • hGrip103 antibody
    • hSpc97 antibody
    • MGC138162 antibody
    • MGC83533 antibody
    • OTTHUMP00000020808 antibody
    • SPBC97 antibody
    • Spc97p antibody
    • Spindle pole body protein Spc97 homolog antibody
    • TUBGCP 2 antibody
    • Tubgcp2 antibody
    • Tubulin gamma complex associated protein 2 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab140225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140225, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

This product has been referenced in:

  • Dráberová E  et al. Overexpression and Nucleolar Localization of ?-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma. J Neuropathol Exp Neurol 74:723-42 (2015). Human . Read more (PubMed: 26079448) »
See 1 Publication for this product

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