Product nameAnti-Tubulin antibody - Loading Control
See all Tubulin primary antibodies
DescriptionRabbit polyclonal to Tubulin - Loading Control
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Zebrafish
Synthetic peptide within Zebrafish Tubulin aa 386-445. The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: Q7ZW01
- Zebrafish eye and brain whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000.|
|IHC-P||1/100 - 1/1000.|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Sequence similaritiesBelongs to the tubulin family.
modificationsUndergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Entrez Gene: 406811 Zebrafish
- Tubulin beta 2b antibody
- Alpha tubulin antibody
- Alpha-tubulin ubiquitous antibody
Paraffin embedded zebrafish tissue stained for Tubulin with ab211116 (1/300) in Immunohistochemical analysis.
All lanes : Anti-Tubulin antibody - Loading Control (ab211116) at 1/10000 dilution
Lane 1 : zebrafish eye whole cell lysate
Lane 2 : zebrafish brain whole cell lysate
Lysates/proteins at 30 µg per lane.
10% SDS PAGE
ab211116 has not yet been referenced specifically in any publications.