Overview

  • Product name

    Anti-Tubulin antibody - Loading Control
    See all Tubulin primary antibodies
  • Description

    Mouse monoclonal to Tubulin - Loading Control
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Rabbit, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Tubulin aa 1-451.
    Database link: P68363

  • General notes

    This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab56676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Tubulin beta 2b antibody
    • Alpha tubulin antibody
    • Alpha-tubulin ubiquitous antibody
    • beta Ib tubulin antibody
    • CDCBM5 antibody
    • CDCBM6 antibody
    • fd02b12 antibody
    • K ALPHA 1 antibody
    • M40 antibody
    • OK/SW-cl.56 antibody
    • TBA1B_HUMAN antibody
    • TUBA1B antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin alpha 1b antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin beta 1b antibody
    • Tubulin beta 2A antibody
    • tubulin beta 2A class IIa antibody
    • Tubulin beta antibody
    • tubulin beta chain antibody
    • tubulin beta class I antibody
    • tubulin beta-1 chain antibody
    • tubulin beta-2A chain antibody
    • tubulin beta-5 chain antibody
    • Tubulin K-alpha-1 antibody
    • tubulin, alpha, ubiquitous antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta polypeptide 2 antibody
    • tubulin, beta polypeptide antibody
    • tubulin, beta, class IIA antibody
    • wu:fd02b12 antibody
    • zgc:55461 antibody
    see all

Images

  • Tubulin antibody (ab56676) at 1ug/lane + A-431 cell lysate at 25ug/lane.

    This image was generated using the ascites version of the product.

  • Tubulin antibody (ab56676) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human prostate.

    This image was generated using the ascites version of the product.

  • ab56676 at 1/500 dilution staining Tubulin in Human Osteosarcoma cells by immunocytochemistry/ Immunofluorescence. The cells were PFA fixed, permeabilized in PBS (0.5% Triton) and blocked in 1% BSA for 1 hour at 25°C. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG at 1/250 dilution, was used as secondary antibody. The primary antibody shows green staining in image.

    This image was generated using the ascites version of the product.

    See Abreview

  • All lanes : Anti-Tubulin antibody - Loading Control (ab56676) at 1 µg/ml (in TPBS for 18 hours at 4°C)

    Lane 1 : Whole tissue lysate of rabbit heart at 20 µg
    Lane 2 : Whole tissue lysate of rabbit heart at 40 µg
    Lane 3 : Whole tissue lysate of rabbit heart at 60 µg
    Lane 4 : Whole tissue lysate of rabbit heart at 80 µg
    Lane 5 : Whole tissue lysate of rabbit heart at 100 µg
    Lane 6 : Whole tissue lysate of rabbit heart at 120 µg

    Secondary
    All lanes : An HRP-conjugated Donkey anti-mouse IgG polyclonal at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 47 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking Step : 5% BSA for 2 hours at 23°C

    This image was generated using the ascites version of the product.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab56676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56676, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Zhao S  et al. High-pressure carbon dioxide pneumoperitoneum induces oxidative stress and mitochondria-associated apoptotic pathway in rabbit kidneys with severe hydronephrosis. Int J Mol Med 43:305-315 (2019). Read more (PubMed: 30431064) »
  • Zhu J  et al. Activation of SK/KCa Channel Attenuates Spinal Cord Ischemia-Reperfusion Injury via Anti-oxidative Activity and Inhibition of Mitochondrial Dysfunction in Rabbits. Front Pharmacol 10:325 (2019). Read more (PubMed: 31001121) »
See all 24 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
Yes - Triton X-100
Specification
Intestine
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Dr. Egi Kardia

Verified customer

Submitted Sep 06 2018

Answer

Thank you for contacting us. This product does not contain any hazardous chemicals and thus does not require an SDS, but we will create one for this product which shows that there are no hazardous components. I will let you know when it is available online and also send it to you as an attachment.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (PC12)
Specification
PC12
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton X100
Blocking step
Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 14 2011

Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
20 µg
Specification
heart
Gel Running Conditions
Reduced Non-Denaturing (Native) (10%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Xun Ai

Verified customer

Submitted Sep 14 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (osteosarcoma cell line)
Loading amount
15 µg
Specification
osteosarcoma cell line
Gel Running Conditions
Reduced Denaturing (page 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Mr. Cesar Camacho

Verified customer

Submitted May 18 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (osteosarcoma cell line)
Specification
osteosarcoma cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - pbs-triton .5%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C

Abcam user community

Verified customer

Submitted May 18 2010

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