Overview

  • Product name
  • Description
    Rabbit polyclonal to TUFM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 25 - 276 of Human TUFM (UniProt ID: P49411).

  • Positive control
    • 293T, H1299 and NIH 3T3 cell lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab155328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
  • Involvement in disease
    Combined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities
    Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • COXPD4 antibody
    • EF Tu antibody
    • EF TuMT antibody
    • EF-Tu antibody
    • EF-TuMT antibody
    • EFTu antibody
    • EFTU_HUMAN antibody
    • EFTuMT antibody
    • Elongation factor Tu antibody
    • Elongation factor Tu mitochondrial antibody
    • mitochondrial antibody
    • P43 antibody
    • Tu translation elongation factor, mitochondrial antibody
    • Tufm antibody
    see all

Images

  • All lanes : Anti-TUFM antibody (ab155328) at 1/1500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Anti-TUFM antibody (ab155328) at 1/1000 dilution + NIH 3T3 cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling TUFM using ab155328 at 1/500 dilution (green). Alpha tubulin filaments were labeled with an Alpha tubulin antibody at 1/2500 dilution (red).

References

This product has been referenced in:
  • Roselló-Lletí E  et al. Heart mitochondrial proteome study elucidates changes in cardiac energy metabolism and antioxidant PRDX3 in human dilated cardiomyopathy. PLoS One 9:e112971 (2014). WB ; Human . Read more (PubMed: 25397948) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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