Anti-TUFM antibody [EPR12796(B)] (ab175199)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-TUFM antibody [EPR12796(B)]
See all TUFM primary antibodies -
DescriptionRabbit monoclonal [EPR12796(B)] to TUFM
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Host speciesRabbit
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Tested applicationsSuitable for: IP, Flow Cyt, ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human TUFM aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P49411 -
Positive control
- HepG2, A431, HeLa and Jurkat whole cell lysate (ab7899)
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR12796(B)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
- Anti-TUFM antibody [EPR12796(B)] (Alexa Fluor® 488) (ab208555)
- Anti-TUFM antibody [EPR12796(B)] (Alexa Fluor® 594) (ab208556)
- Anti-TUFM antibody [EPR12796(B)] (Alexa Fluor® 555) (ab208557)
- Anti-TUFM antibody [EPR12796(B)] (Alexa Fluor® 568) (ab208558)
- Anti-TUFM antibody [EPR12796(B)] (Alexa Fluor® 647) (ab208626)
- Anti-TUFM antibody [EPR12796(B)] (HRP) (ab209019)
- Anti-TUFM antibody [EPR12796(B)] (Alexa Fluor® 405) (ab210032)
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/2500. | |
| ICC/IF | 1/500. For unpurified use at 1/50 - 1/100 |
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| WB | 1/10000 - 1/50000. Predicted molecular weight: 50 kDa. |
Target
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FunctionThis protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
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Involvement in diseaseCombined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry.
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Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
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Cellular localizationMitochondrion.
- Information by UniProt
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Database links
- Entrez Gene: 7284 Human
- Entrez Gene: 233870 Mouse
- Entrez Gene: 293481 Rat
- Omim: 602389 Human
- SwissProt: P49411 Human
- SwissProt: Q8BFR5 Mouse
- SwissProt: P85834 Rat
- Unigene: 12084 Human
see all -
Alternative names
- COXPD4 antibody
- EF Tu antibody
- EF TuMT antibody
see all
Images
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![Western blot - Anti-TUFM antibody [EPR12796(B)] (ab175199)](https://www.abcam.com/ps/products/175/ab175199/Images/ab175199-201554-ab1751991.jpg)
Western blot - Anti-TUFM antibody [EPR12796(B)] (ab175199)All lanes : Anti-TUFM antibody [EPR12796(B)] (ab175199) at 1/10000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : A431 cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)](https://www.abcam.com/ps/products/175/ab175199/Images/ab175199-261224-ab175199-HepG2-500-methanol.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) cells labelling TUFM with purified ab175199 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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![Flow Cytometry - Anti-TUFM antibody [EPR12796(B)] (ab175199)](https://www.abcam.com/ps/products/175/ab175199/Images/ab175199-5-ab175199FC1.jpg)
Flow Cytometry - Anti-TUFM antibody [EPR12796(B)] (ab175199)Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling TUFM (red) with purified ab175199 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)](https://www.abcam.com/ps/products/175/ab175199/Images/ab175199-201553-ab1751992.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)Immunofluorescence analysis of HepG2 cells labeling TUFM with ab175199 at a 1/50 dilution (green). DAPI nuclear staining (blue).
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![Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] (ab175199)](https://www.abcam.com/ps/products/175/ab175199/Images/ab175199-201552-ab1751993.jpg)
Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] (ab175199)Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1X PBS (negative control) using ab175199 at a 1/10 dilution and HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Viale A et al. Oncogene ablation-resistant pancreatic cancer cells depend on mitochondrial function. Nature : (2014). Read more (PubMed: 25119024) »
