Product nameAnti-TUFM antibody [EPR12796(B)]
See all TUFM primary antibodies
DescriptionRabbit monoclonal [EPR12796(B)] to TUFM
Tested applicationsSuitable for: IP, Flow Cyt, ICC/IF, WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TUFM aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P49411
- HepG2, A431, HeLa and Jurkat whole cell lysate (ab7899)
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
For unpurified use at 1/50 - 1/100
|WB||1/10000 - 1/50000. Predicted molecular weight: 50 kDa.|
FunctionThis protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Involvement in diseaseCombined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
- Information by UniProt
- COXPD4 antibody
- EF Tu antibody
- EF TuMT antibody
All lanes : Anti-TUFM antibody [EPR12796(B)] (ab175199) at 1/10000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : A431 cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa
Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) cells labelling TUFM with purified ab175199 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling TUFM (red) with purified ab175199 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunofluorescence analysis of HepG2 cells labeling TUFM with ab175199 at a 1/50 dilution (green). DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1X PBS (negative control) using ab175199 at a 1/10 dilution and HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.