Recombinant Anti-TUFM antibody [EPR12796(B)] (ab175199)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12796(B)] to TUFM
- Suitable for: IP, Flow Cyt, ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-TUFM antibody [EPR12796(B)]
See all TUFM primary antibodies -
Description
Rabbit monoclonal [EPR12796(B)] to TUFM -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human TUFM aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P49411 -
Positive control
- HepG2, A431, HeLa and Jurkat whole cell lysate (ab7899)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12796(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
Flow Cyt |
Human
|
ICC/IF |
Human
|
IHC-P |
Mouse
|
IP |
Human
|
WB |
Human
|
All applications |
Mouse
Rat
|
Application | Abreviews | Notes |
---|---|---|
IP |
1/10 - 1/100.
|
|
Flow Cyt |
1/2500.
|
|
ICC/IF |
1/500.
For unpurified use at 1/50 - 1/100 |
|
WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
|
Notes |
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IP
1/10 - 1/100. |
Flow Cyt
1/2500. |
ICC/IF
1/500. For unpurified use at 1/50 - 1/100 |
WB
1/10000 - 1/50000. Predicted molecular weight: 50 kDa. |
Target
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Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. -
Involvement in disease
Combined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 7284 Human
- Entrez Gene: 233870 Mouse
- Entrez Gene: 293481 Rat
- Omim: 602389 Human
- SwissProt: P49411 Human
- SwissProt: Q8BFR5 Mouse
- SwissProt: P85834 Rat
- Unigene: 12084 Human
see all -
Alternative names
- COXPD4 antibody
- EF Tu antibody
- EF TuMT antibody
see all
Images
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All lanes : Anti-TUFM antibody [EPR12796(B)] (ab175199) at 1/10000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : A431 cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa -
Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) cells labelling TUFM with purified ab175199 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling TUFM (red) with purified ab175199 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunofluorescence analysis of HepG2 cells labeling TUFM with ab175199 at a 1/50 dilution (green). DAPI nuclear staining (blue).
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Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1X PBS (negative control) using ab175199 at a 1/10 dilution and HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab175199 has been referenced in 1 publication.
- Viale A et al. Oncogene ablation-resistant pancreatic cancer cells depend on mitochondrial function. Nature : (2014). PubMed: 25119024