Recombinant
RabMAb

Recombinant Anti-TUFM antibody [EPR12796(B)] (ab175199)

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Overview

  • Product name

    Anti-TUFM antibody [EPR12796(B)]
    See all TUFM primary antibodies

  • Description

    Rabbit monoclonal [EPR12796(B)] to TUFM

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Flow Cyt, ICC/IF, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TUFM aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P49411

  • Positive control

    • HepG2, A431, HeLa and Jurkat whole cell lysate (ab7899)

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab175199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
Flow Cyt 1/2500.
ICC/IF 1/500.

For unpurified use at 1/50 - 1/100
WB 1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

    • Involvement in disease

      Combined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry.

    • Sequence similarities

      Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

    • Cellular localization

      Mitochondrion.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • COXPD4 antibody
      • EF Tu antibody
      • EF TuMT antibody
      • EF-Tu antibody
      • EF-TuMT antibody
      • EFTu antibody
      • EFTU_HUMAN antibody
      • EFTuMT antibody
      • Elongation factor Tu antibody
      • Elongation factor Tu mitochondrial antibody
      • mitochondrial antibody
      • P43 antibody
      • Tu translation elongation factor, mitochondrial antibody
      • Tufm antibody
      see all

    Images

    • Western blot - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      Western blot - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      All lanes : Anti-TUFM antibody [EPR12796(B)] (ab175199) at 1/10000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) cells labelling TUFM with purified ab175199 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Flow Cytometry - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      Flow Cytometry - Anti-TUFM antibody [EPR12796(B)] (ab175199)

      Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling TUFM (red) with purified ab175199 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)

      Immunofluorescence analysis of HepG2 cells labeling TUFM with ab175199 at a 1/50 dilution (green). DAPI nuclear staining (blue).

    • Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] (ab175199)

      Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1X PBS (negative control) using ab175199 at a 1/10 dilution and HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab175199? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-TUFM antibody [EPR12796(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Mouse brain homogenate, Mitochondria from mouse br)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Mouse brain homogenate, Mitochondria from mouse br
    Blocking step
    SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 27 2017

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