RecombinantRabMAb

Recombinant Anti-TUFM antibody [EPR12796(B)] (ab175199)

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Western blot - Anti-TUFM antibody [EPR12796(B)] (ab175199)
  • Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)
  • Flow Cytometry - Anti-TUFM antibody [EPR12796(B)] (ab175199)
  • Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)
  • Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] (ab175199)
  • Anti-TUFM antibody [EPR12796(B)] (ab175199)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12796(B)] to TUFM
  • Suitable for: IP, Flow Cyt, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TUFM antibody [EPR12796(B)]
    See all TUFM primary antibodies

  • Description

    Rabbit monoclonal [EPR12796(B)] to TUFM

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TUFM aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P49411

  • Positive control

    • HepG2, A431, HeLa and Jurkat whole cell lysate (ab7899)

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Mouse
IP
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
IP
1/10 - 1/100.
Flow Cyt
1/2500.
ICC/IF
1/500.

For unpurified use at 1/50 - 1/100
WB (1)
1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
Notes
IP
1/10 - 1/100.
Flow Cyt
1/2500.
ICC/IF
1/500.

For unpurified use at 1/50 - 1/100
WB
1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

    • Involvement in disease

      Combined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry.

    • Sequence similarities

      Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

    • Cellular localization

      Mitochondrion.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • COXPD4 antibody
      • EF Tu antibody
      • EF TuMT antibody
      • EF-Tu antibody
      • EF-TuMT antibody
      • EFTu antibody
      • EFTU_HUMAN antibody
      • EFTuMT antibody
      • Elongation factor Tu antibody
      • Elongation factor Tu mitochondrial antibody
      • mitochondrial antibody
      • P43 antibody
      • Tu translation elongation factor, mitochondrial antibody
      • Tufm antibody
      see all

    Images

    • Western blot - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      Western blot - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      All lanes : Anti-TUFM antibody [EPR12796(B)] (ab175199) at 1/10000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) cells labelling TUFM with purified ab175199 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Flow Cytometry - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      Flow Cytometry - Anti-TUFM antibody [EPR12796(B)] (ab175199)

      Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling TUFM (red) with purified ab175199 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] (ab175199)

      Immunofluorescence analysis of HepG2 cells labeling TUFM with ab175199 at a 1/50 dilution (green). DAPI nuclear staining (blue).

    • Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] (ab175199)
      Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] (ab175199)

      Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1X PBS (negative control) using ab175199 at a 1/10 dilution and HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-TUFM antibody [EPR12796(B)] (ab175199)
      Anti-TUFM antibody [EPR12796(B)] (ab175199)

    References (1)

    Publishing research using ab175199? Please let us know so that we can cite the reference in this datasheet.

    ab175199 has been referenced in 1 publication.

    • Viale A  et al. Oncogene ablation-resistant pancreatic cancer cells depend on mitochondrial function. Nature : (2014). PubMed: 25119024

    Customer reviews and Q&As

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    Western blot abreview for Anti-TUFM antibody [EPR12796(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Mouse brain homogenate, Mitochondria from mouse br)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Mouse brain homogenate, Mitochondria from mouse br
    Blocking step
    SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 27 2017

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