Recombinant Anti-TUFM antibody [EPR12797(B)] (ab173300)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12797(B)] to TUFM
- Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TUFM antibody [EPR12797(B)]
See all TUFM primary antibodies -
Description
Rabbit monoclonal [EPR12797(B)] to TUFM -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TUFM aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P49411 -
Positive control
- HepG2, A431, HeLa, and Jurkat whole cell lysate (ab7899), Human breast and Human colon tissues, A431 cells and Jurkat cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12797(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab173300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/10000 - 1/50000. Detects a band of approximately 43 kDa (predicted molecular weight: 50 kDa). | |
ICC/IF | 1/100 - 1/250. | |
IP | 1/10 - 1/100. | |
Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. -
Involvement in disease
Combined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 7284 Human
- Entrez Gene: 233870 Mouse
- Entrez Gene: 293481 Rat
- Omim: 602389 Human
- SwissProt: P49411 Human
- SwissProt: Q8BFR5 Mouse
- SwissProt: P85834 Rat
- Unigene: 12084 Human
see all -
Alternative names
- COXPD4 antibody
- EF Tu antibody
- EF TuMT antibody
see all
Images
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All lanes : Anti-TUFM antibody [EPR12797(B)] (ab173300) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa -
Immunoprecipitation. ab173300 at 1/10000 staining TUFM immunoprecipitated from HeLa lysate using ab173300 at 1/10.
Lane 2: 1X PBS (negative control).
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Immunofluorescent analysis of A431 cells labeling TUFM with ab173300 at 1/100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] (ab173300)
Immunohistochemical analysis of paraffin embedded Human breast tissue labeling TUFM with ab173300 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] (ab173300)
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TUFM with ab173300 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cytometrical analysis of permeabilized Jurkat cells with ab173300 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
References (2)
ab173300 has been referenced in 2 publications.
- Wang K et al. The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses. Cell Rep 27:2075-2091.e5 (2019). PubMed: 31091447
- Yokokawa T et al. Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle. Physiol Rep 6:e13893 (2018). PubMed: 30369085