Name: Anti-TUFM antibody [EPR12797(B)]
Brand: RabMAb
SKU: ab173300
Rating: 5 (1 reviews)

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Western blot - Anti-TUFM antibody [EPR12797(B)] (ab173300)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12797(B)] to TUFM
Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-TUFM antibody [EPR12797(B)]
See all TUFM primary antibodies
Description

Rabbit monoclonal [EPR12797(B)] to TUFM
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TUFM aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P49411
Positive control
- HepG2, A431, HeLa, and Jurkat whole cell lysate (ab7899), Human breast and Human colon tissues, A431 cells and Jurkat cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12797(B)
Isotype

IgG
Research areas

Alternative Versions
- Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human TUFM protein (His tag) (ab236184)

Our Abpromise guarantee covers the use of ab173300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000 - 1/50000. Detects a band of approximately 43 kDa (predicted molecular weight: 50 kDa).
ICC/IF		1/100 - 1/250.
IP		1/10 - 1/100.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Function

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Involvement in disease

Combined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry.
Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
Cellular localization

Mitochondrion.
Target information above from: UniProt accession P49411 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7284 Human
- Entrez Gene: 233870 Mouse
- Entrez Gene: 293481 Rat
- Omim: 602389 Human
- SwissProt: P49411 Human
- SwissProt: Q8BFR5 Mouse
- SwissProt: P85834 Rat
- Unigene: 12084 Human
- Unigene: 197829 Mouse
- Unigene: 48850 Rat
see all
Alternative names
- COXPD4 antibody
- EF Tu antibody
- EF TuMT antibody
- EF-Tu antibody
- EF-TuMT antibody
- EFTu antibody
- EFTU_HUMAN antibody
- EFTuMT antibody
- Elongation factor Tu antibody
- Elongation factor Tu mitochondrial antibody
- mitochondrial antibody
- P43 antibody
- Tu translation elongation factor, mitochondrial antibody
- Tufm antibody
see all

Western blot - Anti-TUFM antibody [EPR12797(B)] (ab173300)

All lanes : Anti-TUFM antibody [EPR12797(B)] (ab173300) at 1/10000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 50 kDa
Immunoprecipitation - Anti-TUFM antibody [EPR12797(B)] (ab173300)

Immunoprecipitation. ab173300 at 1/10000 staining TUFM immunoprecipitated from HeLa lysate using ab173300 at 1/10.
Lane 2: 1X PBS (negative control).
Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12797(B)] (ab173300)

Immunofluorescent analysis of A431 cells labeling TUFM with ab173300 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] (ab173300)

Immunohistochemical analysis of paraffin embedded Human breast tissue labeling TUFM with ab173300 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] (ab173300)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TUFM with ab173300 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-TUFM antibody [EPR12797(B)] (ab173300)

Flow Cytometrical analysis of permeabilized Jurkat cells with ab173300 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
Anti-TUFM antibody [EPR12797(B)] (ab173300)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet

Publishing research using ab173300? Please let us know so that we can cite the reference in this datasheet.

ab173300 has been referenced in 2 publications.

Wang K et al. The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses. Cell Rep 27:2075-2091.e5 (2019). PubMed: 31091447
Yokokawa T et al. Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle. Physiol Rep 6:e13893 (2018). PubMed: 30369085

Customer reviews and Q&As

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Application

Western blot

Sample

Mouse Cell lysate - whole cell (Hippocampus)

Gel Running Conditions

Reduced Denaturing (gel 10%)

Loading amount

25 µg

Specification

Hippocampus

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 27 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-TUFM antibody [EPR12797(B)] (ab173300)

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (2)

Customer reviews and Q&As

Western blot abreview for Anti-TUFM antibody [EPR12797(B)]