Recombinant
RabMAb

Anti-TUFM antibody [EPR12797(B)] (ab173300)

Overview

  • Product name
    Anti-TUFM antibody [EPR12797(B)]
    See all TUFM primary antibodies
  • Description
    Rabbit monoclonal [EPR12797(B)] to TUFM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TUFM aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P49411

  • Positive control
    • HepG2, A431, HeLa, and Jurkat cell lysates, Human breast and Human colon tissues, A431 cells and Jurkat cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab173300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 43 kDa (predicted molecular weight: 50 kDa).
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100 - 1/250.

Target

  • Function
    This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
  • Involvement in disease
    Combined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities
    Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • COXPD4 antibody
    • EF Tu antibody
    • EF TuMT antibody
    • EF-Tu antibody
    • EF-TuMT antibody
    • EFTu antibody
    • EFTU_HUMAN antibody
    • EFTuMT antibody
    • Elongation factor Tu antibody
    • Elongation factor Tu mitochondrial antibody
    • mitochondrial antibody
    • P43 antibody
    • Tu translation elongation factor, mitochondrial antibody
    • Tufm antibody
    see all

Images

  • All lanes : Anti-TUFM antibody [EPR12797(B)] (ab173300) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 50 kDa

  • Immunoprecipitation. ab173300 at 1/10000 staining TUFM immunoprecipitated from HeLa lysate using ab173300 at 1/10.

    Lane 2: 1X PBS (negative control).

  • Immunofluorescent analysis of A431 cells labeling TUFM with ab173300 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human breast tissue labeling TUFM with ab173300 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TUFM with ab173300 at 1/100.

  • Flow Cytometrical analysis of permeabilized Jurkat cells with ab173300 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

References

ab173300 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hippocampus)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
25 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Jun 27 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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