Recombinant Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12797(B)] to TUFM - BSA and Azide free
- Suitable for: IHC-P, ICC, WB, IP, Flow Cyt
- Reacts with: Rat, Human
Overview
-
Product name
Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free
See all TUFM primary antibodies -
Description
Rabbit monoclonal [EPR12797(B)] to TUFM - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human TUFM aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P49411 -
General notes
Ab249761 is the carrier-free version of ab173300. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab249761 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12797(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
Flow Cyt |
Human
|
IHC-P |
Human
|
IP |
Human
|
WB |
Human
|
All applications |
Mouse
|
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 50 kDa).
|
|
IP |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 50 kDa). |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Target
-
Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. -
Involvement in disease
Combined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. -
Cellular localization
Mitochondrion. - Information by UniProt
-
Database links
- Entrez Gene: 7284 Human
- Entrez Gene: 233870 Mouse
- Entrez Gene: 293481 Rat
- Omim: 602389 Human
- SwissProt: P49411 Human
- SwissProt: Q8BFR5 Mouse
- SwissProt: P85834 Rat
- Unigene: 12084 Human
see all -
Alternative names
- COXPD4 antibody
- EF Tu antibody
- EF TuMT antibody
see all
Images
-
All lanes : Anti-TUFM antibody [EPR12797(B)] (ab173300) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDaThis data was developed using ab173300, the same antibody clone in a different buffer formulation.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)This data was developed using ab173300, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human breast tissue labeling TUFM with ab173300 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)This data was developed using ab173300, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TUFM with ab173300 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab249761 has not yet been referenced specifically in any publications.