RecombinantRabMAb

Recombinant Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)

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Western blot - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
  • Immunoprecipitation - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
  • Immunocytochemistry - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
  • Flow Cytometry - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
  • Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12797(B)] to TUFM - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB, IP, Flow Cyt
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free
    See all TUFM primary antibodies

  • Description

    Rabbit monoclonal [EPR12797(B)] to TUFM - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TUFM aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P49411

  • General notes

    Ab249761 is the carrier-free version of ab173300. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249761 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
IP
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 50 kDa).
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 50 kDa).
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Target

  • Function

    This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

  • Involvement in disease

    Combined oxidative phosphorylation deficiency 4 (COXPD4) [MIM:610678]: A mitochondrial disease resulting in neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Note=The disease is caused by mutations affecting the gene represented in this entry.

  • Sequence similarities

    Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

  • Cellular localization

    Mitochondrion.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • COXPD4 antibody
    • EF Tu antibody
    • EF TuMT antibody
    • EF-Tu antibody
    • EF-TuMT antibody
    • EFTu antibody
    • EFTU_HUMAN antibody
    • EFTuMT antibody
    • Elongation factor Tu antibody
    • Elongation factor Tu mitochondrial antibody
    • mitochondrial antibody
    • P43 antibody
    • Tu translation elongation factor, mitochondrial antibody
    • Tufm antibody
    see all

Images

  • Western blot - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    Western blot - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    All lanes : Anti-TUFM antibody [EPR12797(B)] (ab173300) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 50 kDa



    This data was developed using ab173300, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    Immunoprecipitation - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)

    This data was developed using ab173300, the same antibody clone in a different buffer formulation.

    Immunoprecipitation. ab173300 at 1/10000 staining TUFM immunoprecipitated from HeLa lysate using ab173300 at 1/10.

    Lane 2: 1X PBS (negative control).

  • Immunocytochemistry - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    Immunocytochemistry - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    This data was developed using ab173300, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of A431 cells labeling TUFM with ab173300 at 1/100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    This data was developed using ab173300, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human breast tissue labeling TUFM with ab173300 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    This data was developed using ab173300, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TUFM with ab173300 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    Flow Cytometry - Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    This data was developed using ab173300, the same antibody clone in a different buffer formulation.Flow Cytometrical analysis of permeabilized Jurkat cells with ab173300 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
  • Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)
    Anti-TUFM antibody [EPR12797(B)] - BSA and Azide free (ab249761)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab249761? Please let us know so that we can cite the reference in this datasheet.

ab249761 has not yet been referenced specifically in any publications.

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