Anti-Tuftelin 1 antibody (ab204234)
Key features and details
- Rabbit polyclonal to Tuftelin 1
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tuftelin 1 antibody
See all Tuftelin 1 primary antibodies -
Description
Rabbit polyclonal to Tuftelin 1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human Tuftelin 1 aa 100-200.
Database link: Q9NNX1 -
Positive control
- IHC-P: Human kidney, placenta, skin and small intestine tissue. ICC: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in the mineralization and structural organization of enamel. -
Sequence similarities
Belongs to the tuftelin family. -
Cellular localization
Secreted. Secreted at a very early stage of enamel formation, concentrated at the dentin-enamel junction and tightly bound to the surface of the growing crystallites. - Information by UniProt
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Database links
- Entrez Gene: 282104 Cow
- Entrez Gene: 7286 Human
- Omim: 600087 Human
- SwissProt: P27628 Cow
- SwissProt: Q9NNX1 Human
- Unigene: 489922 Human
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Alternative names
- MGC188530 antibody
- TUFT1 antibody
- TUFT1_HUMAN antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human small intestine tissue labeling Tuftelin 1 with ab204234 at 1/50 dilution.
Moderate cytoplasmic positivity in glandular cells.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of A431 cells labeling Tuftelin 1 with ab204234 at 4 µg/mL.
Fixation/Permeabilization: PFA/Triton X-100.
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Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Tuftelin 1 with ab204234 at 1/50 dilution.
Moderate cytoplasmic positivity in squamous epithelial cells.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Tuftelin 1 with ab204234 at 1/50 dilution.
Moderate cytoplasmic positivity in trophoblastic cells.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Tuftelin 1 with ab204234 at 1/50 dilution.
Strong cytoplasmic positivity in cells in tubules.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204234 has not yet been referenced specifically in any publications.