Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8615] to TUG
- Suitable for: WB, IP, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-TUG antibody [EPR8615]
See all TUG primary antibodies
DescriptionRabbit monoclonal [EPR8615] to TUG
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in human TUG (Q9BZE9).
- K562, 293T, and SW480 cell lysates for Western Blot and Human colon tissue for IHC-P.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C.
Dissociation constant (KD)KD = 2.77 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 60 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface.
Tissue specificityUbiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.
Involvement in diseaseNote=A chromosomal aberration involving ASPSCR1 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with TFE3 forms a ASPSCR1-TFE3 fusion protein.
Note=A chromosomal aberration involving ASPSCR1 has been found in two patients with of papillary renal cell carcinoma. Translocation t(X;17)(p11.2;q25).
Sequence similaritiesContains 1 UBX domain.
Cellular localizationEndomembrane system.
- Information by UniProt
- Alveolar soft part sarcoma chromosomal region candidate gene 1 protein antibody
- Alveolar soft part sarcoma chromosome region candidate 1 (human) antibody
- Alveolar soft part sarcoma locus antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TUG knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab131237 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab131237 was shown to specifically react with TUG when TUG knockout samples were used. Wild-type and TUG knockout samples were subjected to SDS-PAGE. ab131237 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab131237, at 1/100, staining TUG in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-TUG antibody [EPR8615] (ab131237) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131237 has not yet been referenced specifically in any publications.