Overview

  • Product name

    Anti-TUG antibody [EPR8615]
    See all TUG primary antibodies
  • Description

    Rabbit monoclonal [EPR8615] to TUG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in human TUG (Q9BZE9).

  • Positive control

    • K562, 293T, and SW480 cell lysates for Western Blot and Human colon tissue for IHC-P.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface.
    • Tissue specificity

      Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.
    • Involvement in disease

      Note=A chromosomal aberration involving ASPSCR1 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with TFE3 forms a ASPSCR1-TFE3 fusion protein.
      Note=A chromosomal aberration involving ASPSCR1 has been found in two patients with of papillary renal cell carcinoma. Translocation t(X;17)(p11.2;q25).
    • Sequence similarities

      Contains 1 UBX domain.
    • Cellular localization

      Endomembrane system.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alveolar soft part sarcoma chromosomal region candidate gene 1 protein antibody
      • Alveolar soft part sarcoma chromosome region candidate 1 (human) antibody
      • Alveolar soft part sarcoma locus antibody
      • ASPC antibody
      • ASPC1_HUMAN antibody
      • ASPCR 1 antibody
      • ASPCR1 antibody
      • ASPL antibody
      • ASPS antibody
      • ASPSCR 1 antibody
      • Aspscr1 antibody
      • FLJ45380 antibody
      • RCC 17 antibody
      • RCC17 antibody
      • renal cell carcinoma gene on chromosome 17 antibody
      • renal cell carcinoma papillary 17 antibody
      • Renal papillary cell carcinoma protein 17 antibody
      • Tether containing a UBX domain for GLUT4 antibody
      • Tether containing UBX domain for GLUT4 antibody
      • TUG antibody
      • UBX domain containing protein 9 antibody
      • UBX domain protein 9 antibody
      • UBX domain-containing protein 9 antibody
      • UBXD 9 antibody
      • UBXD9 antibody
      • UBXN 9 antibody
      • UBXN9 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: TUG knockout HAP1 cell lysate (20 µg)
      Lane 3: NIH/3T3 cell lysate (20 µg)
      Lane 4: HEK293 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab131237 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab131237 was shown to specifically react with TUG when TUG knockout samples were used. Wild-type and TUG knockout samples were subjected to SDS-PAGE. ab131237 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776  secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • ab131237, at 1/100, staining TUG in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry.
    • All lanes : Anti-TUG antibody [EPR8615] (ab131237) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab131237 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.05% Triton X100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Apr 30 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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