Recombinant Anti-TUG antibody [EPR8616] (ab131217)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8616] to TUG
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TUG antibody [EPR8616]
See all TUG primary antibodies -
Description
Rabbit monoclonal [EPR8616] to TUG -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, 293T, and SW480 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8616 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 60 kDa.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 60 kDa. |
Target
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Function
Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface. -
Tissue specificity
Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas. -
Involvement in disease
Note=A chromosomal aberration involving ASPSCR1 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with TFE3 forms a ASPSCR1-TFE3 fusion protein.
Note=A chromosomal aberration involving ASPSCR1 has been found in two patients with of papillary renal cell carcinoma. Translocation t(X;17)(p11.2;q25). -
Sequence similarities
Contains 1 UBX domain. -
Cellular localization
Endomembrane system. - Information by UniProt
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Database links
- Entrez Gene: 79058 Human
- Entrez Gene: 68938 Mouse
- Entrez Gene: 691026 Rat
- Omim: 606236 Human
- SwissProt: Q9BZE9 Human
- SwissProt: Q8VBT9 Mouse
- Unigene: 298351 Human
- Unigene: 294020 Mouse
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Alternative names
- Alveolar soft part sarcoma chromosomal region candidate gene 1 protein antibody
- Alveolar soft part sarcoma chromosome region candidate 1 (human) antibody
- Alveolar soft part sarcoma locus antibody
see all
Images
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TUG knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab131217 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab131217 was shown to specifically react with TUG when TUG knockout samples were used. Wild-type and TUG knockout samples were subjected to SDS-PAGE. ab131217 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-TUG antibody [EPR8616] (ab131217) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131217 has not yet been referenced specifically in any publications.