Key features and details
- Rabbit polyclonal to TULP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TULP1 antibody
DescriptionRabbit polyclonal to TULP1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment containing a sequence corresponding to a region within amino acids 281-520 of Human TULP1 (NP_003313).
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji whole cell lysates; Breast CA
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/100 - 1/250.|
FunctionRequired for normal development of photoreceptor synapses. Required for normal photoreceptor function and for long-term survival of photoreceptor cells. Interacts with cytoskeleton proteins and may play a role in protein transport in photoreceptor cells (By similarity). Binds lipids, especially phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, phosphatidylinositol 3,4-bisphosphate, phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-bisphosphate, phosphatidylserine and phosphatidic acid (in vitro). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages.
Involvement in diseaseDefects in TULP1 are the cause of retinitis pigmentosa type 14 (RP14) [MIM:600132]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP14 inheritance is autosomal recessive.
Defects in TULP1 are the cause of Leber congenital amaurosis type 15 (LCA15) [MIM:613843]. LCA15 is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus.
Sequence similaritiesBelongs to the TUB family.
Cellular localizationCytoplasm. Cell membrane. Secreted. Cell junction > synapse. Detected at synapses between photoreceptor cells and second-order neurons. Does not have a cleavable signal peptide and is secreted by an alternative pathway.
- Information by UniProt
- RP14 antibody
- Tubby like protein 1 antibody
- Tubby related protein 1 (Tubby like protein 1) antibody
All lanes : Anti-TULP1 antibody (ab97281) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : Molt-4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS PAGE
Immunohistochemical analysis of TULP1 in paraffin-embedded Breast CA, using ab97281 at 1/100 dilution.
ab97281 has been referenced in 1 publication.
- Rodriguez AS et al. Tubby-like protein 1 (Tulp1) is a target of microRNA-134 and is down-regulated in experimental epilepsy. Int J Physiol Pathophysiol Pharmacol 9:178-187 (2017). WB, IHC-P . PubMed: 29348794