Key features and details
- Rabbit polyclonal to TULP3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TULP3 antibody
See all TULP3 primary antibodies
DescriptionRabbit polyclonal to TULP3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: HEK-293 overexpressing TULP3 with C-terminal myc-DDK tag, cell lysate. IHC-P: Human colon tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionNegatively regulates SHH signal transduction (By similarity). Probably functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to phosphorylated inositide lipids.
Tissue specificityExpressed at high levels in testis, ovaries, thyroid, and spinal chord.
Sequence similaritiesBelongs to the TUB family.
Cellular localizationNucleus. Cell membrane. Cell projection > cilium. Cytoplasm. Secreted. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway (By similarity). Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha.
- Information by UniProt
- MGC45295 antibody
- Tubby like protein 3 antibody
- Tubby related protein 3 antibody
Paraffin-embedded human colon tissue stained for TULP3 using ab254692 at 1/20 dilution in immunohistochemical analysis.
All lanes : Anti-TULP3 antibody (ab254692) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T cell lysate
Lane 2 : HEK-293 overexpressing TULP3 with C-terminal myc-DDK tag, cell lysate.
Predicted band size: 50 kDa
ab254692 has not yet been referenced specifically in any publications.