Overview

  • Product name
    Tunicamycin, Protein glycosylation inhibitor.
  • Description
    Antibiotic. Protein glycosylation inhibitor.
  • Biological description
    Nucleoside antibiotic that inhibits protein glycosylation. Inhibits GlcNAc phosphotransferase (GPT) and inhibits the transfer of N-acetylglucosamine-1-phosphate from UDP-N-acetylglucosamine to dolichol phosphate in the first step of glycoprotein synthesis. Inhibits N-linked glycosylation and blocks the formation of N-glycosidic protein-carbohydrate linkages. Active in vitro against Gram-positive bacteria, yeasts, fungi and viruses.
  • Purity
    > 98%
  • General notes

    This compound is a mixture of the homologs of tunicamycin: A, B, C and D which vary in the carbon chain length (n=8 to 11)

  • CAS Number
    11089-65-9
  • Chemical structure
    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab120296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • All lanes : Anti-ATF-4 antibody [EPR18111] (ab184909) at 1/1000 dilution

    Lane 1 : Untreated HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate (control)
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) treated with 5 µM MG-132 (ab141003) and 3 µg/ml tunicamycin (ab120296) for 6 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22095285).

References

This product has been referenced in:
See all 6 Publications for this product

Customer reviews and Q&As

Answer

Thank you very much for your call last week and for your patience while I have been in touch with our biochemical scientists regarding your enquiry.

We quote solubility in DMSO of 50mM and believe it's quite easily dissolved at room temperature – no special procedures have been recorded. We also run the NMR in DMSO so we are confident that there won't be an issue.

We do fully guarantee ab120296 to work as stated on the datasheet, and if you have any problems at all with it we will be happy to refund or replace it for you.
I hope that this information will be useful, but if you have any further questions or need anything else, please let me know and I'll be happy to help.

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