Anti-FLK1 antibody [Clone 10] (ab24313)
Key features and details
- Mouse monoclonal [Clone 10] to FLK1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-FLK1 antibody [Clone 10] -
Description
Mouse monoclonal [Clone 10] to FLK1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant fragment corresponding to Saccharomyces cerevisiae FLK1 aa 1-200.
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Positive control
- Mav108 cells expressing recombinant FLK1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
Clone 10 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 78 kDa).
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Notes |
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WB
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 78 kDa). |
Target
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Relevance
Yeast TUP1 Ssn6p repressor complex represents a novel paradigm for transcriptional repression and for the role of chromatin in repression. TUP1 and SSN6 are involved in repression of several diverse families of genes in yeast, including cell type specific genes regulated by the alpha2 and a1/alpha2 repressors. Ssn6 Tupl interacts with class I histone deacetylases required for repression, and recruitment of yeast Tuplp Ssn6p repressor is associated with localised decreases in histone acetylation. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 850445 Saccharomyces cerevisiae
- SwissProt: P16649 Saccharomyces cerevisiae
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Alternative names
- FLK1 antibody
- Flocculation suppressor protein antibody
- Repressor AER2 antibody
see all
Images
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All lanes : Anti-FLK1 antibody [Clone 10] (ab24313)
All lanes : Saccharomyces cerevisiae whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Sheep anti-Mouse polyclonal HRP conjugate at 100 %
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Datasheets and documents
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Datasheet download
References (1)
ab24313 has been referenced in 1 publication.
- Mei B et al. The regulatory mechanism and biological significance of the Snail-miR590-VEGFR-NRP1 axis in the angiogenesis, growth and metastasis of gastric cancer. Cell Death Dis 11:241 (2020). PubMed: 32303680