Key features and details
- Rabbit polyclonal to TUSC3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TUSC3 antibody
DescriptionRabbit polyclonal to TUSC3
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Monkey
Synthetic peptide corresponding to Human TUSC3 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q13454
- IHC-P: Human colon cancer and testis tissue. ICC/IF: HeLa cells. WB: SK-BR-3, HeLa, HepG2 and MDA-MB-231 whole cell lysates.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230520 in the following tested applications.
|WB||1/500 - 1/1000. Predicted molecular weight: 40 kDa.|
|ICC/IF||1/100 - 1/500.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceTUSC3 belongs to the OST3/OST6 family of proteins and may be involved in N-glycosylation through its association with N-oligosaccharyl transferase. The TUSC3 gene is a candidate tumour supressor.
Cellular localizationEndoplasmic reticulum membrane; Multi-pass membrane protein.
- D8S1992 antibody
- MGC13453 antibody
- N33 antibody
All lanes : Anti-TUSC3 antibody (ab230520) at 1/500 dilution
Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line)
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma)
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line)
Lane 4 : MDA-MB-231 (Human breast adenocarcinoma cell line)
Developed using the ECL technique.
Predicted band size: 40 kDa
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for TUSC3 with ab230520 at a 1/100 dilution in immunohistochemical analysis.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ab230520 at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for TUSC3 using ab230520 at a dilution of 1/100 in ICC/IF.
Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Formalin-fixed, paraffin-embedded human testis tissue stained for TUSC3 with ab230520 at a 1/100 dilution in immunohistochemical analysis.
ab230520 has not yet been referenced specifically in any publications.