Anti-TUSC3 antibody (ab230520)
Key features and details
- Rabbit polyclonal to TUSC3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TUSC3 antibody -
Description
Rabbit polyclonal to TUSC3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Monkey -
Immunogen
Synthetic peptide corresponding to Human TUSC3 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q13454 -
Positive control
- IHC-P: Human colon cancer and testis tissue. ICC/IF: HeLa cells. WB: SK-BR-3, HeLa, HepG2 and MDA-MB-231 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 40 kDa.
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ICC/IF |
1/100 - 1/500.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 40 kDa. |
ICC/IF
1/100 - 1/500. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
TUSC3 belongs to the OST3/OST6 family of proteins and may be involved in N-glycosylation through its association with N-oligosaccharyl transferase. The TUSC3 gene is a candidate tumour supressor. -
Cellular localization
Endoplasmic reticulum membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 615007 Cow
- Entrez Gene: 7991 Human
- Omim: 601385 Human
- SwissProt: Q32L57 Cow
- SwissProt: Q13454 Human
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Alternative names
- D8S1992 antibody
- MGC13453 antibody
- N33 antibody
see all
Images
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All lanes : Anti-TUSC3 antibody (ab230520) at 1/500 dilution
Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line)
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma)
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line)
Lane 4 : MDA-MB-231 (Human breast adenocarcinoma cell line)
Developed using the ECL technique.
Predicted band size: 40 kDa -
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for TUSC3 with ab230520 at a 1/100 dilution in immunohistochemical analysis.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ab230520 at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for TUSC3 using ab230520 at a dilution of 1/100 in ICC/IF.
Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Formalin-fixed, paraffin-embedded human testis tissue stained for TUSC3 with ab230520 at a 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab230520 has been referenced in 2 publications.
- Sun F et al. TUSC3 inhibits cell proliferation and invasion in cervical squamous cell carcinoma via suppression of the AKT signalling pathway. J Cell Mol Med 26:1629-1642 (2022). PubMed: 35137520
- Kong L et al. Downregulation of microRNA-320a inhibits proliferation and induces apoptosis of retinoblastoma cells via targeting TUSC3. Exp Ther Med 20:9 (2020). PubMed: 32934674