Overview

  • Product name

  • Description

    Rabbit polyclonal to TUSC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human TUSC3 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: Q13454

  • Positive control

    • IHC-P: Human colon cancer and testis tissue. ICC/IF: HeLa cells. WB: SK-BR-3, HeLa, HepG2 and MDA-MB-231 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab230520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ICC/IF 1/100 - 1/500.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    TUSC3 belongs to the OST3/OST6 family of proteins and may be involved in N-glycosylation through its association with N-oligosaccharyl transferase. The TUSC3 gene is a candidate tumour supressor.
  • Cellular localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • Database links

  • Alternative names

    • D8S1992 antibody
    • MGC13453 antibody
    • N33 antibody
    • OST3A antibody
    • Protein N33 antibody
    • Putative prostate cancer tumor suppressor antibody
    • Tumor suppressor candidate 3 antibody
    • TUSC 3 antibody
    see all

Images

  • All lanes : Anti-TUSC3 antibody (ab230520) at 1/500 dilution

    Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line)
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma)
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line)
    Lane 4 : MDA-MB-231 (Human breast adenocarcinoma cell line)

    Developed using the ECL technique.

    Predicted band size: 40 kDa

  • Formalin-fixed, paraffin-embedded human colon cancer tissue stained for TUSC3 with ab230520 at a 1/100 dilution in immunohistochemical analysis.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ab230520 at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for TUSC3 using ab230520 at a dilution of 1/100 in ICC/IF.

    Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Formalin-fixed, paraffin-embedded human testis tissue stained for TUSC3 with ab230520 at a 1/100 dilution in immunohistochemical analysis.

References

ab230520 has not yet been referenced specifically in any publications.

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