Overview

  • Product name
  • Description
    Goat polyclonal to TUSC3
  • Host species
    Goat
  • Specificity
    Expected to recognise both reported isoforms of TUSC3 (NP_006756.2; NP_839952.1).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Macaque monkey
  • Immunogen

    Synthetic peptide:

    PQRQCSVCRQAN

    , corresponding to internal sequence amino acids 95-106 of Human TUSC3 isoform a and b (NP_006756.2; NP_839952.1)

  • Positive control
    • Human liver lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/1000.
    WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 40 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Anti-TUSC3 antibody (ab77600) at 1 µg/ml + Human liver lysate at 35 µg

      Predicted band size: 40 kDa
      Observed band size: 37 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.



      Both bands were successfully blocked by incubation with the immunising peptide.

    References

    This product has been referenced in:
    • Burgermeister E  et al. Epigenetic silencing of tumor suppressor candidate 3 confers adverse prognosis in early colorectal cancer. Oncotarget 8:84714-84728 (2017). Read more (PubMed: 29156678) »
    • Peng Y  et al. TUSC3 induces autophagy in human non-small cell lung cancer cells through Wnt/ß-catenin signaling. Oncotarget 8:52960-52974 (2017). Read more (PubMed: 28881786) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77600.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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