Overview

  • Product name

  • Description

    Goat polyclonal to TWEAK
  • Host species

    Goat
  • Tested applications

    Suitable for: Neutralising, WB, Indirect ELISA, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Highly pure (>98%) recombinant Human TWEAK.

Properties

Applications

Our Abpromise guarantee covers the use of ab109824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use a concentration of 0.1 - 0.2 µg/ml. to yield one-half maximal inhibition inhibition [ND50] of the biological activity of Human TWEAK (200 ng/ml)
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant Human TWEAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. ab109824 allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TWEAK (using 100 µl/well antibody solution).
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to TWEAK (Biotin) (ab83437). ab109824 in conjunction with ab83437 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TWEAK (using 100 µl/well antibody solution).

Target

  • Function

    Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.
  • Tissue specificity

    Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • APO 3 ligand antibody
    • APO 3L antibody
    • APO3 ligand antibody
    • APO3/DR3 ligand antibody
    • APO3L antibody
    • DR3LG antibody
    • MGC129581 antibody
    • MGC20669 antibody
    • secreted form antibody
    • TNF-related weak inducer of apoptosis antibody
    • TNF12_HUMAN antibody
    • TNFSF 12 antibody
    • Tnfsf12 antibody
    • TNFSF12 protein antibody
    • Tumor necrosis factor (ligand) superfamily member 12 antibody
    • Tumor necrosis factor ligand superfamily member 12 antibody
    • Tumor necrosis factor superfamily member 12 antibody
    • TWEAK antibody
    • UNQ181/PRO207 antibody
    see all

References

This product has been referenced in:

  • Bover LC  et al. A previously unrecognized protein-protein interaction between TWEAK and CD163: potential biological implications. J Immunol 178:8183-94 (2007). Read more (PubMed: 17548657) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

The immunogen sequence for antibody ab108924 is a E.coli expressed recombinant protein with sequence MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH. This sequence is 92% similar with mouse sequence so it is most likely that ab109824 will cross react with the mouse species as well.

This anybody is tested in Functional assays on human cells so it is guaranteed. We however haven't tested this ab in mouse so human cells will be good positive control to try if you plan to use this product with mouse.

If you opt to use this ab in mouse then please do not forget to get a Abtrial discount code from me. Click the link for more details; https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up