• Product name

  • Description

    Rabbit polyclonal to TWEAK
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Recombinant full length TWEAK (Human)

  • Positive control

    • HT29 (Human colon adenocarcinoma grade II cell line) whole cell lysate



Our Abpromise guarantee covers the use of ab37170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 27 kDa). TWEAK may migrate at aberrant poitions in SDS-PAGE.
ICC/IF Use a concentration of 10 µg/ml.


  • Function

    Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.
  • Tissue specificity

    Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational

    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • APO 3 ligand antibody
    • APO 3L antibody
    • APO3 ligand antibody
    • APO3/DR3 ligand antibody
    • APO3L antibody
    • DR3LG antibody
    • MGC129581 antibody
    • MGC20669 antibody
    • secreted form antibody
    • TNF-related weak inducer of apoptosis antibody
    • TNF12_HUMAN antibody
    • TNFSF 12 antibody
    • Tnfsf12 antibody
    • TNFSF12 protein antibody
    • Tumor necrosis factor (ligand) superfamily member 12 antibody
    • Tumor necrosis factor ligand superfamily member 12 antibody
    • Tumor necrosis factor superfamily member 12 antibody
    • TWEAK antibody
    • UNQ181/PRO207 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse brain tissue staining TWEAK with ab37170 at 20µg/ml
  • Western Blot of HeLa cell lysate labeling TWEAK with Anti-TWEAK antibody (ab37170) at (1) 1 and (2) 2µg/ml.
  • All lanes : Anti-TWEAK antibody (ab37170) at 1 µg/ml

    Lane 1 : Recombinant human TWEAK at 0.005 µg
    Lane 2 : Recombinant human TWEAK at 0.025 µg
    Lane 3 : Recombinant human TWEAK at 0.05 µg

    Predicted band size: 27 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

    TWEAK migrates at aberrant positions in SDS-PAGE; hence the observed band is different from the predicted molecular weight of 27 kDa.
  • ICC/IF image of ab37170 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37170, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Padrão AI  et al. Exercise training protects against cancer-induced cardiac remodeling in an animal model of urothelial carcinoma. Arch Biochem Biophys 645:12-18 (2018). WB ; Rat . Read more (PubMed: 29548774) »
  • Howard L  et al. TWE-PRIL reverse signalling suppresses sympathetic axon growth and tissue innervation. Development 145:N/A (2018). Read more (PubMed: 30337376) »
See all 9 Publications for this product

Customer reviews and Q&As


Thank you for sending along the western blot images. I just have a few additional questions: 1. Are the samples of TWEAK transfected cell extracts or are these samples purified recombinant TWEAK protein or are they untransfected cell lysates or tissue lysates? 2. Any idea why the high molecular weight bands (all at the same molecular weight) are staining so intensely? 3. Do both blots contain identical samples loaded in the identical order, where the only difference is the antibody used for detection? Thanks!

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