Question (24190) | Anti-TWEAK antibody (ab37170)

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Question

Here is a powerpoint of 2 westerns. Now, I feel like an idiot because I don't have a film where I ran the recombinant with the ab86287. I was mad and threw it out! BUT, I swear that the samples were running at 27 and the recombinant was running at 37 like it was supposed to. From the slide, you can see ab37170 running with my samples at 37 KDa with the recombinant but with ab86287 there is a signal at around 27 KDa and again, the recombinant was running at 37 and on another film there were no bands at 37 (that I threw away).

Answer

Thank you for sending along the western blot images. I just have a few additional questions: 1. Are the samples of TWEAK transfected cell extracts or are these samples purified recombinant TWEAK protein or are they untransfected cell lysates or tissue lysates? 2. Any idea why the high molecular weight bands (all at the same molecular weight) are staining so intensely? 3. Do both blots contain identical samples loaded in the identical order, where the only difference is the antibody used for detection? Thanks!