Anti-TWEAK antibody [CARL-1] (ab234269)
Key features and details
- Mouse monoclonal [CARL-1] to TWEAK
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG3
Overview
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Product name
Anti-TWEAK antibody [CARL-1]
See all TWEAK primary antibodies -
Description
Mouse monoclonal [CARL-1] to TWEAK -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human TWEAK. (Human TWEAK-transfected 2PK-3 cells).
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Positive control
- Flow Cyt: CD255 transfectants.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: 99% Tris buffered saline -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purity: > 95% (by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
CARL-1 -
Isotype
IgG3 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 1 - 4 µg/ml. |
Target
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Function
Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. -
Tissue specificity
Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8742 Human
- Omim: 602695 Human
- SwissProt: O43508 Human
- Unigene: 54673 Human
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Alternative names
- APO 3 ligand antibody
- APO 3L antibody
- APO3 ligand antibody
see all
Images
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Flow cytometry analysis of l5178Y cell suspensions stained using ab234269 (concentration in sample 3 μg/ml) showing separation of CD255 transfected l5178Y cells (red-filled) from CD255 nontransfected (WT) l5178Y cells (black-dashed).
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Flow cytometric analysis of human CD255 transfectants, labeling TWEAK with ab234269 at 9 µg/ml. Gated on singlets.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab234269 has not yet been referenced specifically in any publications.