Anti-TWEAKR/FN14 antibody (ab62496)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-TWEAKR/FN14 antibody
See all TWEAKR/FN14 primary antibodies -
Description
Rabbit polyclonal to TWEAKR/FN14 -
Host species
Rabbit -
Specificity
This antibody reacts with TWEAKR/FN14
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Tested applications
Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type corresponding to Human TWEAKR/FN14.
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Positive control
- HepG2 cell lysate, 293 cells and human liver tissue
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General notes
This product was previously labelled as TWEAKR
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab62496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 14 kDa). | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 2.5 µg/ml. |
Target
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Function
Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. -
Tissue specificity
Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. -
Sequence similarities
Contains 1 TNFR-Cys repeat. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 51330 Human
- Omim: 605914 Human
- SwissProt: Q9NP84 Human
- Unigene: 355899 Human
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Alternative names
- CD 266 antibody
- CD266 antibody
- CD266 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody (ab62496)Immunohistochemistry of TWEAKR/FN14 in human liver tissue with ab62496 antibody at 2.5 µg/ml.
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Western blot - Anti-TWEAKR/FN14 antibody (ab62496)All lanes : Anti-TWEAKR/FN14 antibody (ab62496) at 1 µg/ml
Lane 1 : mock transfected 293 cells
Lane 2 : Fn14-GPI transfected 293 cells
Lysates/proteins at 15 µg per lane.
Predicted band size: 14 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted? -
Western blot - Anti-TWEAKR/FN14 antibody (ab62496) -
Immunocytochemistry/ Immunofluorescence - Anti-TWEAKR/FN14 antibody (ab62496)ICC/IF image of ab62496 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62496, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-TWEAKR/FN14 antibody (ab62496)Immunofluorescence of Fn14 in Human Liver cells using ab62496 at 20 ug/ml.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Affò S et al. Transcriptome analysis identifies TNF superfamily receptors as potential therapeutic targets in alcoholic hepatitis. Gut : (2012). IHC-P ; Human . Read more (PubMed: 22637703) »
