Anti-TWEAKR/FN14 antibody (ab62496)
Key features and details
- Rabbit polyclonal to TWEAKR/FN14
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TWEAKR/FN14 antibody
See all TWEAKR/FN14 primary antibodies -
Description
Rabbit polyclonal to TWEAKR/FN14 -
Host species
Rabbit -
Specificity
This antibody reacts with TWEAKR/FN14
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type corresponding to Human TWEAKR/FN14.
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Positive control
- HepG2 cell lysate, 293 cells and human liver tissue
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General notes
This product was previously labelled as TWEAKR
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab62496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 14 kDa). | |
| IHC-P | Use a concentration of 2.5 µg/ml. |
Target
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Function
Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. -
Tissue specificity
Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. -
Sequence similarities
Contains 1 TNFR-Cys repeat. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 51330 Human
- Omim: 605914 Human
- SwissProt: Q9NP84 Human
- Unigene: 355899 Human
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Alternative names
- CD 266 antibody
- CD266 antibody
- CD266 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody (ab62496)
Immunohistochemistry of TWEAKR/FN14 in human liver tissue with ab62496 antibody at 2.5 µg/ml.
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Western blot - Anti-TWEAKR/FN14 antibody (ab62496)All lanes : Anti-TWEAKR/FN14 antibody (ab62496) at 1 µg/ml
Lane 1 : mock transfected 293 cells
Lane 2 : Fn14-GPI transfected 293 cells
Lysates/proteins at 15 µg per lane.
Predicted band size: 14 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted? -
Western blot - Anti-TWEAKR/FN14 antibody (ab62496) -
Immunocytochemistry/ Immunofluorescence - Anti-TWEAKR/FN14 antibody (ab62496)ICC/IF image of ab62496 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62496, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-TWEAKR/FN14 antibody (ab62496)Immunofluorescence of Fn14 in Human Liver cells using ab62496 at 20 ug/ml.
Protocols
Datasheets and documents
References (1)
ab62496 has been referenced in 1 publication.
- Affò S et al. Transcriptome analysis identifies TNF superfamily receptors as potential therapeutic targets in alcoholic hepatitis. Gut : (2012). IHC-P ; Human . PubMed: 22637703
