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    tweakrfn14-antibody-ab62496.pdf

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Cardiovascular Angiogenesis Growth Factors Other
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Anti-TWEAKR/FN14 antibody (ab62496)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody (ab62496)
  • Western blot - Anti-TWEAKR/FN14 antibody (ab62496)
  • Western blot - Anti-TWEAKR/FN14 antibody (ab62496)
  • Immunocytochemistry/ Immunofluorescence - Anti-TWEAKR/FN14 antibody (ab62496)
  • Immunocytochemistry/ Immunofluorescence - Anti-TWEAKR/FN14 antibody (ab62496)

Key features and details

  • Rabbit polyclonal to TWEAKR/FN14
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-TWEAKR/FN14 antibody
    See all TWEAKR/FN14 primary antibodies
  • Description

    Rabbit polyclonal to TWEAKR/FN14
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with TWEAKR/FN14

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human TWEAKR/FN14.

  • Positive control

    • HepG2 cell lysate, 293 cells and human liver tissue
  • General notes

     This product was previously labelled as TWEAKR

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Cancer
    • Growth factors
    • TNF

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HEK293 whole cell lysate (ab7902)

Applications

Our Abpromise guarantee covers the use of ab62496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 14 kDa).
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function

    Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
  • Tissue specificity

    Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
  • Sequence similarities

    Contains 1 TNFR-Cys repeat.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q9NP84 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51330 Human
    • Omim: 605914 Human
    • SwissProt: Q9NP84 Human
    • Unigene: 355899 Human
    • Alternative names

      • CD 266 antibody
      • CD266 antibody
      • CD266 antigen antibody
      • FGF inducible 14 antibody
      • FGF-inducible 14 antibody
      • Fibroblast growth factor inducible immediate early response protein 14 antibody
      • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
      • FN 14 antibody
      • FN14 antibody
      • TNFRSF 12A antibody
      • TNFRSF12A antibody
      • TNR12_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 12A antibody
      • TWEAK R antibody
      • Tweak receptor antibody
      • Tweak-receptor antibody
      • TweakR antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody (ab62496)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody (ab62496)

      Immunohistochemistry of TWEAKR/FN14 in human liver tissue with ab62496 antibody at 2.5 µg/ml.

    • Western blot - Anti-TWEAKR/FN14 antibody (ab62496)
      Western blot - Anti-TWEAKR/FN14 antibody (ab62496)
      All lanes : Anti-TWEAKR/FN14 antibody (ab62496) at 1 µg/ml

      Lane 1 : mock transfected 293 cells
      Lane 2 : Fn14-GPI transfected 293 cells

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 14 kDa
      Observed band size: 33 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-TWEAKR/FN14 antibody (ab62496)
      Western blot - Anti-TWEAKR/FN14 antibody (ab62496)
    • Immunocytochemistry/ Immunofluorescence - Anti-TWEAKR/FN14 antibody (ab62496)
      Immunocytochemistry/ Immunofluorescence - Anti-TWEAKR/FN14 antibody (ab62496)
      ICC/IF image of ab62496 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62496, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
    • Immunocytochemistry/ Immunofluorescence - Anti-TWEAKR/FN14 antibody (ab62496)
      Immunocytochemistry/ Immunofluorescence - Anti-TWEAKR/FN14 antibody (ab62496)
      Immunofluorescence of Fn14 in Human Liver cells using ab62496 at 20 ug/ml.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab62496? Please let us know so that we can cite the reference in this datasheet.

    ab62496 has been referenced in 1 publication.

    • Affò S  et al. Transcriptome analysis identifies TNF superfamily receptors as potential therapeutic targets in alcoholic hepatitis. Gut : (2012). IHC-P ; Human . PubMed: 22637703

    Customer reviews and Q&As

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