Overview

  • Product name

  • Description

    Rabbit polyclonal to TWEAKR/FN14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human TWEAKR/FN14 (internal sequence).
    Database link: NM_016639.1

  • Positive control

    • liver
  • General notes

     This product was previously labelled as TWEAKR

     

Properties

  • Form

    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50 - 1/200.

Target

  • Function

    Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
  • Tissue specificity

    Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
  • Sequence similarities

    Contains 1 TNFR-Cys repeat.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 266 antibody
    • CD266 antibody
    • CD266 antigen antibody
    • FGF inducible 14 antibody
    • FGF-inducible 14 antibody
    • Fibroblast growth factor inducible immediate early response protein 14 antibody
    • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
    • FN 14 antibody
    • FN14 antibody
    • TNFRSF 12A antibody
    • TNFRSF12A antibody
    • TNR12_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 12A antibody
    • TWEAK R antibody
    • Tweak receptor antibody
    • Tweak-receptor antibody
    • TweakR antibody
    see all

Images

  • Immunohistochemical analysis of TWEAKR/FN14 expression in paraffin embedded formalin fixed liver using 1/100 ab85089. Note membrane staining.

  • ICC/IF image of ab85089 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85089, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab85089 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your kind email. I am sorry to hear that the antibody is still giving you problems. Before sending the free of charge replacement, I just wanted to confirm that it is ab109365 you would like to receive, right? https://www.abcam.com/tweakr-antibody-epr3179-ab109365.html Please let me know and I'll arrange the shipment I look forward to hear back and wish you as well Happy Holidays!

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Answer

Thank you for your email. I apologize for the long delay in getting back to you - I was on vacation and my colleagues are not able to check my direct email, unfortunately. Do you still have problems with emailing technical@abcam.com? It might be worthwile to try one more time. I took a look at the images you sent: Yes, there seems to be some nuclear staining as well as nice plasma membrane staining. My suggestions would be to dilute the primary antibody further as the nuclear staining might be due to too much primary antibody. In addition or as alternative, using less secondary antibody might help as well. Further, increasing the blocking time could help improve the results, too. Should none of this have the desired effect, it might be best to try a different TWEAKR antibody, such as ab109365. Please let me know if these suggestions are of help. If not, please let me know if trying another antibody would help you. Further options would be a credit or refund. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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