Recombinant Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free (ab275381)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23427-30] to TWEAKR/FN14 - BSA and Azide free
- Suitable for: Flow Cyt, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free
See all TWEAKR/FN14 primary antibodies -
Description
Rabbit monoclonal [EPR23427-30] to TWEAKR/FN14 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IPmore details
Unsuitable for: ICC/IF,IHC-P or WB -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: NIH/3T3 cells (either starved for 8h then treated with 30ng/ml PMA for 16h or starved for 8h then treated with 10ng/ml FGF2 for 16h). IP: C2C12 whole cell lysate.
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General notes
ab275381 is the carrier-free version of ab275375.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23427-30 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Target
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Function
Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. -
Tissue specificity
Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. -
Sequence similarities
Contains 1 TNFR-Cys repeat. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 27279 Mouse
- SwissProt: Q9CR75 Mouse
- Unigene: 28518 Mouse
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Alternative names
- CD 266 antibody
- CD266 antibody
- CD266 antigen antibody
see all
Images
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This data was developed using ab275375, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast) (starved for 8h then treated with 10ng/ml FGF2 for 16h) (Red) / Untreated control (Green) cells labelling TWEAKR/FN14 with ab275375 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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This data was developed using ab275375, the same antibody clone in a different buffer formulation.
TWEAKR/FN14 was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast) whole cell lysate with ab275375 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275375 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: C2C12 (mouse myoblasts myoblast) whole cell lysate 10 ug
Lane 2: ab275375 IP in C2C12 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275375 in C2C12 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 50 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID:17383968).
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This data was developed using ab275375, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast) (starved for 8h then treated with 30ng/ml PMA for 16h) (Red) / Untreated control (Green) cells labelling TWEAKR/FN14 with ab275375 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab275381 has not yet been referenced specifically in any publications.