Recombinant
RabMAb

Recombinant Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

Overview

  • Product name
    Anti-TWEAKR/FN14 antibody [EPR3179]
    See all TWEAKR/FN14 primary antibodies
  • Description
    Rabbit monoclonal [EPR3179] to TWEAKR/FN14
  • Host species
    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications
    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TWEAKR/FN14 (C terminal). The exact sequence is proprietary.

  • Positive control
    • HT-29, NIH/3T3, L6, C2C12, HUVEC and HT-1080 cell lysates; Human uterus tissue, human bladder carcinoma and human cardiac muscle.
  • General notes

     This product was previously labelled as TWEAKR

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 24467773
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP 1/40.

For unpurified use at 1/10.

IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
    • Tissue specificity
      Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
    • Sequence similarities
      Contains 1 TNFR-Cys repeat.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 266 antibody
      • CD266 antibody
      • CD266 antigen antibody
      • FGF inducible 14 antibody
      • FGF-inducible 14 antibody
      • Fibroblast growth factor inducible immediate early response protein 14 antibody
      • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
      • FN 14 antibody
      • FN14 antibody
      • TNFRSF 12A antibody
      • TNFRSF12A antibody
      • TNR12_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 12A antibody
      • TWEAK R antibody
      • Tweak receptor antibody
      • Tweak-receptor antibody
      • TweakR antibody
      see all

    Images

    • ab109365, at 1/50 dilution, staining TWEAKR/FN14 in Human uterus tissue by Immunohistochemistry.

    • Immunohistochemical analysis of paraffin-embedded human bladder carcinoma sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

      Lane 1 : HUVEC (human umbilical vein/vascular endothelium) whole cell lysate
      Lane 2 : HT-1080 (human fibrosarcoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14-17 kDa
      why is the actual band size different from the predicted?



      Blocking/Diluting buffer 5% NFDM /TBST
    • All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
      Lane 2 : L6 (rat skeletal muscle) whole cell lysate
      Lane 3 : C2C12 (mouse muscle) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14-17 kDa why is the actual band size different from the predicted?



      Blocking/Diluting buffer 5% NFDM /TBST
    • ab109365 at 1/40 immunoprecipitating TWEAKR/FN14 in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).

      Lane 1 (input): HUVEC whole cell lysate 10ug

      Lane 2 (+): ab109365 + HUVEC whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109365 in HUVEC whole cell lysate

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

      Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : HT-1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 14 kDa

    References

    This product has been referenced in:
    See all 11 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6
    Permeabilization
    No
    Specification
    skin tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin

    Abcam user community

    Verified customer

    Submitted Jul 16 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered normal formalin

    Abcam user community

    Verified customer

    Submitted Jul 16 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH6
    Permeabilization
    No
    Specification
    bone
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin

    Abcam user community

    Verified customer

    Submitted Jul 16 2016

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