RecombinantRabMAb

Recombinant Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
  • Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
  • Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3179] to TWEAKR/FN14 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free
    See all TWEAKR/FN14 primary antibodies

  • Description

    Rabbit monoclonal [EPR3179] to TWEAKR/FN14 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HT-29, NIH/3T3, L6, C2C12, HUVEC and HT-1080 cell lysates; Human uterus tissue, human bladder carcinoma and human cardiac muscle.

  • General notes

    ab271881 is the carrier-free version of ab109365.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
IP
Use at an assay dependent concentration.

For unpurified use at 1/10.

 
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

 
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
IP
Use at an assay dependent concentration.

For unpurified use at 1/10.

 
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

 
Application notes
Is unsuitable for ICC.

Target

  • Function

    Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.

  • Tissue specificity

    Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.

  • Sequence similarities

    Contains 1 TNFR-Cys repeat.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 266 antibody
      • CD266 antibody
      • CD266 antigen antibody
      • FGF inducible 14 antibody
      • FGF-inducible 14 antibody
      • Fibroblast growth factor inducible immediate early response protein 14 antibody
      • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
      • FN 14 antibody
      • FN14 antibody
      • TNFRSF 12A antibody
      • TNFRSF12A antibody
      • TNR12_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 12A antibody
      • TWEAK R antibody
      • Tweak receptor antibody
      • Tweak-receptor antibody
      • TweakR antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)

      ab109365, at 1/50 dilution, staining TWEAKR/FN14 in Human uterus tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109365).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)

      Immunohistochemical analysis of paraffin-embedded human bladder carcinoma sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109365).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)

      Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109365).
    • Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
      Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)

      ab109365 at 1/40 immunoprecipitating TWEAKR/FN14 in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).

      Lane 1 (input): HUVEC whole cell lysate 10ug

      Lane 2 (+): ab109365 + HUVEC whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109365 in HUVEC whole cell lysate

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

      Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109365).
    • Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
      Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab271881? Please let us know so that we can cite the reference in this datasheet.

    ab271881 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab271881.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com