Recombinant Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)
Overview
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Product name
Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free
See all TWEAKR/FN14 primary antibodies -
Description
Rabbit monoclonal [EPR3179] to TWEAKR/FN14 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, IHC-Frmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TWEAKR/FN14 (C terminal). The exact sequence is proprietary.
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Positive control
- HT-29, NIH/3T3, L6, C2C12, HUVEC and HT-1080 cell lysates; Human uterus tissue, human bladder carcinoma and human cardiac muscle.
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General notes
This product was previously labelled as TWEAKR
ab271881 is a PBS-only buffer format of ab109365. Please refer to ab109365 for recommended dilutions, protocols, and image data.
This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This antibody is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3179 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab271881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. | |
| IP | Use at an assay dependent concentration. For unpurified use at 1/10.
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| IHC-P | Use at an assay dependent concentration. See IHC antigen retrieval protocols.The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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| IHC-Fr | Use at an assay dependent concentration. |
Target
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Function
Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. -
Tissue specificity
Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. -
Sequence similarities
Contains 1 TNFR-Cys repeat. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 51330 Human
- Entrez Gene: 27279 Mouse
- Entrez Gene: 302965 Rat
- Omim: 605914 Human
- SwissProt: Q9NP84 Human
- SwissProt: Q9CR75 Mouse
- Unigene: 355899 Human
- Unigene: 28518 Mouse
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Alternative names
- CD 266 antibody
- CD266 antibody
- CD266 antigen antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)](https://www.abcam.com/ps/products/271/ab271881/Images/ab271881-1-ab109365122128antitweakrantibodyepr3179immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)ab109365, at 1/50 dilution, staining TWEAKR/FN14 in Human uterus tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109365).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)](https://www.abcam.com/ps/products/271/ab271881/Images/ab271881-3-ab109365266767antitweakrantibodyepr3179immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)Immunohistochemical analysis of paraffin-embedded human bladder carcinoma sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109365).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)](https://www.abcam.com/ps/products/271/ab271881/Images/ab271881-2-ab109365266766antitweakrantibodyepr3179immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109365).
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![Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)](https://www.abcam.com/ps/products/271/ab271881/Images/ab271881-4-ab109365266799antitweakrantibodyepr3179immunoprecipitation.jpg)
Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] - BSA and Azide free (ab271881)ab109365 at 1/40 immunoprecipitating TWEAKR/FN14 in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).
Lane 1 (input): HUVEC whole cell lysate 10ug
Lane 2 (+): ab109365 + HUVEC whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109365 in HUVEC whole cell lysate
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109365).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References
ab271881 has not yet been referenced specifically in any publications.
