RecombinantRabMAb

Recombinant Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)

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Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)
  • Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3179] to TWEAKR/FN14 - Low endotoxin, Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free
    See all TWEAKR/FN14 primary antibodies

  • Description

    Rabbit monoclonal [EPR3179] to TWEAKR/FN14 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    (internal sequence).

  • Positive control

    • HT-29, NIH/3T3, L6, C2C12, HUVEC and HT-1080 cell lysates; Human uterus tissue, human bladder carcinoma and human cardiac muscle.

  • General notes

    ab228481 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product was previously labelled as TWEAKR

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab228481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC.

    • Target

    • Function

      Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.

    • Tissue specificity

      Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.

    • Sequence similarities

      Contains 1 TNFR-Cys repeat.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 266 antibody
        • CD266 antibody
        • CD266 antigen antibody
        • FGF inducible 14 antibody
        • FGF-inducible 14 antibody
        • Fibroblast growth factor inducible immediate early response protein 14 antibody
        • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
        • FN 14 antibody
        • FN14 antibody
        • TNFRSF 12A antibody
        • TNFRSF12A antibody
        • TNR12_HUMAN antibody
        • Tumor necrosis factor receptor superfamily member 12A antibody
        • TWEAK R antibody
        • Tweak receptor antibody
        • Tweak-receptor antibody
        • TweakR antibody
        see all

      Images

      • Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)
        Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)

        ab109365 at 1/40 immunoprecipitating TWEAKR/FN14 in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).

        Lane 1 (input): HUVEC whole cell lysate 10ug

        Lane 2 (+): ab109365 + HUVEC whole cell lysate.

        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109365 in HUVEC whole cell lysate

        VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

        Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109365).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)

        Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109365).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)

        This IHC data was generated using the same anti-TWEAKR/FN14 antibody clone, EPR3179, in a different buffer formulation (cat# ab109365).

        ab109365, at 1/50 dilution, staining TWEAKR/FN14 in Human uterus tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)

        This IHC data was generated using the same anti-TWEAKR/FN14 antibody clone, EPR3179, in a different buffer formulation (cat# ab109365).

        Immunohistochemical analysis of paraffin-embedded human bladder carcinoma sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

      • Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)
        Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (ab228481)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab228481? Please let us know so that we can cite the reference in this datasheet.

      ab228481 has not yet been referenced specifically in any publications.

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