Recombinant
RabMAb

Recombinant Anti-TWEAKR/FN14 antibody [SP134] - C-terminal (ab227650)

Overview

  • Product name
    Anti-TWEAKR/FN14 antibody [SP134] - C-terminal
    See all TWEAKR/FN14 primary antibodies
  • Description
    Rabbit monoclonal [SP134] to TWEAKR/FN14 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Frog
  • Immunogen

    Synthetic peptide within Human TWEAKR/FN14 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9NP84

  • Positive control
    • WB: Calu3 cell lysate. IHC-P: Human kidney, lung, prostate, pancreas, renal cell carcinoma, lung adenocarcinoma, pancreatic adenocarcinoma grade I-III, ACHN RCC xenograft, Calu3 xenograft and 22RV1 prostate Ca xenograft tissues. Flow Cyt: HT-29 cells.
  • General notes

     This product was previously labelled as TWEAKR

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

For antigen retrieval: boil tissue sections in 1mM EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

WB 1/25. Predicted molecular weight: 14 kDa.
Flow Cyt 1/100.

Target

  • Function
    Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
  • Tissue specificity
    Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
  • Sequence similarities
    Contains 1 TNFR-Cys repeat.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 266 antibody
    • CD266 antibody
    • CD266 antigen antibody
    • FGF inducible 14 antibody
    • FGF-inducible 14 antibody
    • Fibroblast growth factor inducible immediate early response protein 14 antibody
    • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
    • FN 14 antibody
    • FN14 antibody
    • TNFRSF 12A antibody
    • TNFRSF12A antibody
    • TNR12_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 12A antibody
    • TWEAK R antibody
    • Tweak receptor antibody
    • Tweak-receptor antibody
    • TweakR antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for TWEAKR/FN14 using ab227650 at 1/100 dilution in immunohistochemical analysis.

  • Anti-TWEAKR/FN14 antibody [SP134] - C-terminal (ab227650) at 1/25 dilution + Calu3 cell lysate

    Predicted band size: 14 kDa

  • Flow cytometric analysis of HT-29 (human colorectal adenocarcinoma cell line) cell line labeling TWEAKR/FN14 with ab227650 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).

  • Formalin-fixed, paraffin-embedded human ACHN RCC xenograft tissue stained for TWEAKR/FN14 using ab227650 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for TWEAKR/FN14 using ab227650 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human Calu3 xenograft tissue stained for TWEAKR/FN14 using ab227650 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human 22RV1 prostate Ca xenograft tissue stained for TWEAKR/FN14 using ab227650 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TWEAKR/FN14 using ab227650 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung tissue stained for TWEAKR/FN14 using ab227650 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma grade III tissue stained for TWEAKR/FN14 using ab227650 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreas tissue stained for TWEAKR/FN14 using ab227650 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma grade I-II tissue stained for TWEAKR/FN14 using ab227650 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for TWEAKR/FN14 using ab227650 at 1/100 dilution in immunohistochemical analysis.

References

ab227650 has not yet been referenced specifically in any publications.

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