Anti-TWF1/Twinfilin-1 antibody (ab154725)
Key features and details
- Rabbit polyclonal to TWF1/Twinfilin-1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-TWF1/Twinfilin-1 antibody
See all TWF1/Twinfilin-1 primary antibodies -
Description
Rabbit polyclonal to TWF1/Twinfilin-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment within Human TWF1/Twinfilin-1 aa 92-338. The exact sequence is proprietary.
Database link: Q12792 -
Positive control
- WB: 293T, A431, H1299, HeLaS3 and NIH-3T3 whole cell lysates. ICC/IF: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. -
Tissue specificity
Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. -
Sequence similarities
Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
Contains 2 ADF-H domains. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts. - Information by UniProt
-
Database links
- Entrez Gene: 506683 Cow
- Entrez Gene: 5756 Human
- Entrez Gene: 19230 Mouse
- Entrez Gene: 315265 Rat
- Omim: 610932 Human
- SwissProt: Q56JV6 Cow
- SwissProt: Q12792 Human
- SwissProt: Q91YR1 Mouse
see all -
Alternative names
- A6 antibody
- A6 protein tyrosine kinase antibody
- MGC105817 antibody
see all
Images
-
Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 40 kDa10% SDS PAGE.
-
Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 40 kDa10% SDS PAGE.
-
Immunofluorescent analysis, by confocal microscopy, of paraformaldehyde-fixed HeLa cells labeling TWF1/Twinfilin-1 with ab154725 at 1/500 dilution (green). Alpha-tubulin filaments were labeled in red.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab154725 has been referenced in 1 publication.
- Gyvyte U et al. The Role of miR-375-3p and miR-200b-3p in Gastrointestinal Stromal Tumors. Int J Mol Sci 21:N/A (2020). PubMed: 32708220