Overview

  • Product name
  • Description
    Rabbit polyclonal to TWF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment within Human TWF1 aa 92-338. The exact sequence is proprietary.
    Database link: Q12792

  • Positive control
    • WB: 293T, A431, H1299, HeLaS3 and NIH-3T3 whole cell lysates. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.
  • Tissue specificity
    Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.
  • Sequence similarities
    Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
    Contains 2 ADF-H domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.
  • Information by UniProt
  • Database links
  • Alternative names
    • A6 antibody
    • A6 protein tyrosine kinase antibody
    • MGC105817 antibody
    • MGC23788 antibody
    • MGC41876 antibody
    • Protein A6 antibody
    • Protein tyrosine kinase 9 antibody
    • PTK9 antibody
    • PTK9 protein tyrosine kinase 9 antibody
    • TWF 1 antibody
    • twf1 antibody
    • TWF1_HUMAN antibody
    • Twinfilin 1 antibody
    • Twinfilin actin binding protein, homolog 1 antibody
    • Twinfilin antibody
    • Twinfilin, actin-binding protein, homolog 1 (Drosophila) antibody
    • Twinfilin, Drosophila, homolog of, 1 antibody
    • Twinfilin-1 antibody
    see all

Images

  • Anti-TWF1 antibody (ab154725) at 1/5000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE.

  • Anti-TWF1 antibody (ab154725) at 1/5000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE.

  • Immunofluorescent analysis, by confocal microscopy, of paraformaldehyde-fixed HeLa cells labeling TWF1 with ab154725 at 1/500 dilution (green). Alpha-tubulin filaments were labeled in red.

References

ab154725 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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