Anti-TWF1/Twinfilin-1 antibody (ab154725)

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Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
  • Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
  • Immunocytochemistry/ Immunofluorescence - Anti-TWF1/Twinfilin-1 antibody (ab154725)

Key features and details

  • Rabbit polyclonal to TWF1/Twinfilin-1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-TWF1/Twinfilin-1 antibody
    See all TWF1/Twinfilin-1 primary antibodies

  • Description

    Rabbit polyclonal to TWF1/Twinfilin-1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow

  • Immunogen

    Recombinant fragment within Human TWF1/Twinfilin-1 aa 92-338. The exact sequence is proprietary.
    Database link: Q12792

  • Positive control

    • WB: 293T, A431, H1299, HeLaS3 and NIH-3T3 whole cell lysates. ICC/IF: HeLa cells.

  • General notes

     This product was previously labelled as TWF1

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

  • Tissue specificity

    Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.

  • Sequence similarities

    Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
    Contains 2 ADF-H domains.

  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues.

  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • A6 antibody
    • A6 protein tyrosine kinase antibody
    • MGC105817 antibody
    • MGC23788 antibody
    • MGC41876 antibody
    • Protein A6 antibody
    • Protein tyrosine kinase 9 antibody
    • PTK9 antibody
    • PTK9 protein tyrosine kinase 9 antibody
    • TWF 1 antibody
    • twf1 antibody
    • TWF1_HUMAN antibody
    • Twinfilin 1 antibody
    • Twinfilin actin binding protein, homolog 1 antibody
    • Twinfilin antibody
    • Twinfilin, actin-binding protein, homolog 1 (Drosophila) antibody
    • Twinfilin, Drosophila, homolog of, 1 antibody
    • Twinfilin-1 antibody
    see all

Images

  • Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
    Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
    Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE.

  • Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
    Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
    Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE.

  • Immunocytochemistry/ Immunofluorescence - Anti-TWF1/Twinfilin-1 antibody (ab154725)
    Immunocytochemistry/ Immunofluorescence - Anti-TWF1/Twinfilin-1 antibody (ab154725)

    Immunofluorescent analysis, by confocal microscopy, of paraformaldehyde-fixed HeLa cells labeling TWF1/Twinfilin-1 with ab154725 at 1/500 dilution (green). Alpha-tubulin filaments were labeled in red.

References (1)

Publishing research using ab154725? Please let us know so that we can cite the reference in this datasheet.

ab154725 has been referenced in 1 publication.

  • Gyvyte U  et al. The Role of miR-375-3p and miR-200b-3p in Gastrointestinal Stromal Tumors. Int J Mol Sci 21:N/A (2020). PubMed: 32708220

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