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  1. Link

    twf1twinfilin-1-antibody-ab154725.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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Anti-TWF1/Twinfilin-1 antibody (ab154725)

  • Datasheet
  • SDS
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Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
  • Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
  • Immunocytochemistry/ Immunofluorescence - Anti-TWF1/Twinfilin-1 antibody (ab154725)

Key features and details

  • Rabbit polyclonal to TWF1/Twinfilin-1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human TWF1/Twinfilin-1 protein (ab116148)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TWF1/Twinfilin-1 antibody
    See all TWF1/Twinfilin-1 primary antibodies
  • Description

    Rabbit polyclonal to TWF1/Twinfilin-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment within Human TWF1/Twinfilin-1 aa 92-338. The exact sequence is proprietary.
    Database link: Q12792

  • Positive control

    • WB: 293T, A431, H1299, HeLaS3 and NIH-3T3 whole cell lysates. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as TWF1

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A-431 whole cell lysate (ab30132)
    • NIH/3T3 whole cell lysate (ab7179)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human TWF1/Twinfilin-1 protein (ab116148)
    • Recombinant Human TWF1/Twinfilin-1 protein (ab131676)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.
  • Tissue specificity

    Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.
  • Sequence similarities

    Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
    Contains 2 ADF-H domains.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.
  • Target information above from: UniProt accession Q12792 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 506683 Cow
    • Entrez Gene: 5756 Human
    • Entrez Gene: 19230 Mouse
    • Entrez Gene: 315265 Rat
    • Omim: 610932 Human
    • SwissProt: Q56JV6 Cow
    • SwissProt: Q12792 Human
    • SwissProt: Q91YR1 Mouse
    • SwissProt: Q5RJR2 Rat
    • Unigene: 5944 Cow
    • Unigene: 189075 Human
    • Unigene: 309867 Mouse
    • Unigene: 162971 Rat
    see all
  • Alternative names

    • A6 antibody
    • A6 protein tyrosine kinase antibody
    • MGC105817 antibody
    • MGC23788 antibody
    • MGC41876 antibody
    • Protein A6 antibody
    • Protein tyrosine kinase 9 antibody
    • PTK9 antibody
    • PTK9 protein tyrosine kinase 9 antibody
    • TWF 1 antibody
    • twf1 antibody
    • TWF1_HUMAN antibody
    • Twinfilin 1 antibody
    • Twinfilin actin binding protein, homolog 1 antibody
    • Twinfilin antibody
    • Twinfilin, actin-binding protein, homolog 1 (Drosophila) antibody
    • Twinfilin, Drosophila, homolog of, 1 antibody
    • Twinfilin-1 antibody
    see all

Images

  • Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
    Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
    Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE.

  • Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
    Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
    Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE.

  • Immunocytochemistry/ Immunofluorescence - Anti-TWF1/Twinfilin-1 antibody (ab154725)
    Immunocytochemistry/ Immunofluorescence - Anti-TWF1/Twinfilin-1 antibody (ab154725)

    Immunofluorescent analysis, by confocal microscopy, of paraformaldehyde-fixed HeLa cells labeling TWF1/Twinfilin-1 with ab154725 at 1/500 dilution (green). Alpha-tubulin filaments were labeled in red.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab154725? Please let us know so that we can cite the reference in this datasheet.

ab154725 has been referenced in 1 publication.

  • Gyvyte U  et al. The Role of miR-375-3p and miR-200b-3p in Gastrointestinal Stromal Tumors. Int J Mol Sci 21:N/A (2020). PubMed: 32708220

Customer reviews and Q&As

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