Anti-TWF1/Twinfilin-1 antibody (ab154725)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TWF1/Twinfilin-1 antibody
See all TWF1/Twinfilin-1 primary antibodies -
Description
Rabbit polyclonal to TWF1/Twinfilin-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment within Human TWF1/Twinfilin-1 aa 92-338. The exact sequence is proprietary.
Database link: Q12792 -
Positive control
- WB: 293T, A431, H1299, HeLaS3 and NIH-3T3 whole cell lysates. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as TWF1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 40 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. -
Tissue specificity
Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. -
Sequence similarities
Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
Contains 2 ADF-H domains. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts. - Information by UniProt
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Database links
- Entrez Gene: 506683 Cow
- Entrez Gene: 5756 Human
- Entrez Gene: 19230 Mouse
- Entrez Gene: 315265 Rat
- Omim: 610932 Human
- SwissProt: Q56JV6 Cow
- SwissProt: Q12792 Human
- SwissProt: Q91YR1 Mouse
see all -
Alternative names
- A6 antibody
- A6 protein tyrosine kinase antibody
- MGC105817 antibody
see all
Images
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Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 40 kDa10% SDS PAGE.
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Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 40 kDa10% SDS PAGE.
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Immunocytochemistry/ Immunofluorescence - Anti-TWF1/Twinfilin-1 antibody (ab154725)Immunofluorescent analysis, by confocal microscopy, of paraformaldehyde-fixed HeLa cells labeling TWF1/Twinfilin-1 with ab154725 at 1/500 dilution (green). Alpha-tubulin filaments were labeled in red.
References
ab154725 has not yet been referenced specifically in any publications.
