Key features and details
- Rabbit polyclonal to TWF1/Twinfilin-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TWF1/Twinfilin-1 antibody
See all TWF1/Twinfilin-1 primary antibodies
DescriptionRabbit polyclonal to TWF1/Twinfilin-1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment (His-tag) corresponding to Human TWF1/Twinfilin-1 aa 1-252. Expressed in E.coli. N-terminal tag.
MSHQTGIQASEDVKEIFARARNGKYRLLKISIENEQLVIGSYSQPSDSWD KDYDSFVLPLLEDKQPCYILFRLDSQNAQGYEWIFIAWSPDHSHVRQKML YAATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAA EEELRQIKINEVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNY VQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYL ES
Database link: Q12792-1
- IHC-P: Human brain tissue. WB: Recombinant human TWF1/Twinfilin-1 protein and pig mammary gland tissue lysate.
This product was previously labelled as TWF1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionActin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.
Tissue specificityExpressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.
Sequence similaritiesBelongs to the actin-binding proteins ADF family. Twinfilin subfamily.
Contains 2 ADF-H domains.
modificationsPhosphorylated on serine and threonine residues.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.
- Information by UniProt
- A6 antibody
- A6 protein tyrosine kinase antibody
- MGC105817 antibody
Formalin-fixed, paraffin-embedded human brain tissue stained for TWF1/Twinfilin-1 using ab233129 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Anti-TWF1/Twinfilin-1 antibody (ab233129) at 3 µg/ml + Pig mammary gland tissue lysate
HRP-conjugated goat anti-rabbit IgG (polyclonal) at 0.2 µg/ml
Anti-TWF1/Twinfilin-1 antibody (ab233129) at 2 µg/ml + Recombinant human TWF1/Twinfilin-1 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233129 has not yet been referenced specifically in any publications.