Anti-TWF1/Twinfilin-1 antibody (ab241129)
Key features and details
- Rabbit polyclonal to TWF1/Twinfilin-1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TWF1/Twinfilin-1 antibody
See all TWF1/Twinfilin-1 primary antibodies -
Description
Rabbit polyclonal to TWF1/Twinfilin-1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis -
Immunogen
Synthetic peptide within Human TWF1/Twinfilin-1 aa 1-50. The exact sequence is proprietary.
Database link: Q12792 -
Positive control
- WB: HeLa, HEK-293T and Jurkat cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000.
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Notes |
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WB
1/2000 - 1/10000. |
Target
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Function
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. -
Tissue specificity
Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. -
Sequence similarities
Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
Contains 2 ADF-H domains. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts. - Information by UniProt
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Database links
- Entrez Gene: 506683 Cow
- Entrez Gene: 5756 Human
- Entrez Gene: 19230 Mouse
- Entrez Gene: 315265 Rat
- Entrez Gene: 447307 Xenopus laevis
- Entrez Gene: 496960 Xenopus tropicalis
- Omim: 610932 Human
- SwissProt: Q56JV6 Cow
see all -
Alternative names
- A6 antibody
- A6 protein tyrosine kinase antibody
- MGC105817 antibody
see all
Images
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All lanes : Anti-TWF1/Twinfilin-1 antibody (ab241129) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 3 secondsPrepared using NETN lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab241129 has not yet been referenced specifically in any publications.